白细胞介素-17对子宫内膜癌细胞株HEC-1-B基质金属蛋白酶-2,-9基因表达及肿瘤细胞侵袭转移的影响  

Effects of Interleukin-17 on the Expression of Matric Metalloproteinases-2,-9 in Endometrial Cell Line HEC-1-B and Metastasis of Tumor Cell

作　　者：侯倩男[1] 赖婷[1] 郄明蓉[1] 

机构地区：[1]四川大学华西第二医院妇产科,四川成都610041

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第2期90-93,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：四川省科技厅科研项目(04JY029-077-2)~~

摘　　要：目的探讨白细胞介素(interleukin,IL)-17对子宫内膜癌细胞株HEC-1-B基质金属蛋白酶(matric metalloproteinases,MMP)-2,-9基因表达及肿瘤细胞侵袭转移能力的影响。方法选择对数生长期人子宫内膜癌细胞株HEC-1-B,在无血清培养基中加入白细胞介素-17终浓度分别为1 ng/mL,10 ng/mL,100 ng/mL和无血清培养(空白对照)进行干扰处理后,分别纳入干扰组(干扰组1、干扰组2、干扰组3)及对照组。采用免疫荧光细胞化学方法检测子宫内膜癌细胞株HEC-1-B是否表达白细胞介素-17特异性受体C(IL-17RC)蛋白。采用逆转录荧光定量PCR法检测不同浓度白细胞介素-17对子宫内膜癌细胞株HEC-1-B基质金属蛋白酶-2,-9 mRNA基因表达水平的影响。采用transwell小室检测不同浓度白细胞介素-17干预后,子宫内膜癌细胞株HEC-1-B侵袭转移能力的变化。结果人子宫内膜癌细胞株HEC-1-B高表达特异性白细胞介素-17受体C。白细胞介素-17各浓度干扰组(干扰组1,干扰组2,干扰组3)与对照组比较,干扰组基质金属蛋白酶-9 mRNA表达增加,差异有显著意义(P<0.01),且各浓度干扰组表现呈浓度依赖;而干扰组基质金属蛋白酶-2 mRNA表达无明显变化;干扰组穿膜细胞数明显增多,与对照组比较,差异有显著意义(P<0.01),且各浓度干扰组表现呈浓度依赖;而干扰组2与干扰组3穿膜细胞数较干扰组1增多,差异有显著意义(P<0.05)。结论白细胞介素-17能增强基质金属蛋白酶-9基因表达,促进子宫内膜癌细胞侵袭转移。Objective To investigate the expression of matric metalloproteinases （MMP）-2, -9 and the invasiveness effect of human carcinoma of endometrial cell line HEC-1-B by interleukin （IL）-17. Methods An intracellular domain of interleukin-17 receptor （IL-17RC） protein was detected by immunofluorescence techniques. After endometrial cell line HEC-1-B were treated with different concentrations of interleukin-17 （1 ng/mL, 10 ng/mL, 100 ng/mL and blank control）, they were divided into research groups（research group 1, research group 2, research group 3） and control group. The expression of metalloproteinases -2, -9 mRNA level, invasiveness were detected by RT-PCR and transwell. Results Interleukin-17 effectively increased the expression of matric metalloproteinases-9 mRNA level, even the invasiveness of tumor increased significantly（P〈0.01）. And the effect was concentration-dependant to some extent. Conclusion Interleukin-17 upregulate metalloproteinases-9 expression of human carcinoma of endometrial line HEC-1-B and promote tumor metastasis.

关 键 词：子宫内膜癌细胞株HEC-1-B 白细胞介素-17 基质金属蛋白酶 

分 类 号：R737.33[医药卫生—肿瘤]