卵巢肿瘤组织中血管内皮生长因子mRNA的表达及其临床意义  

Expression of Vascular Endothelial Growth Factor mRNA in Ovarian Neoplasm and Its Clinical Significance

作　　者：王丽梅[1] 刘晓艳[1] 辛楠[1] 尚丽新[1] 

机构地区：[1]北京军区总医院妇产科,北京100700

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第2期94-97,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)mRNA亚型在卵巢肿瘤中表达及其临床意义。方法选择2000年1月至2004年6月在本院妇科住院手术治疗的54例确诊为卵巢恶性肿瘤患者为研究对象。其中,上皮性癌组织为41例,非上皮性肿瘤组织为13例,纳入研究组。选择同期在本院手术治疗的良性卵巢囊肿组织为14例,及正常卵巢组织为16例,纳入对照组。采用逆转录多聚酶链反应(reverse transcriptase-polymerase chain reaction,RT-PCR)非变性聚丙烯酰胺凝胶(polyacrylamide gel,PAG)银染法检测标本中VEGF mRNA亚型表达。采用F8因子(FⅧ-RA)标记标本中微血管密度(microvessel density,MVD)。两组患者年龄及其他内科疾病史等比较,差异无显著意义(P>0.05)。本研究遵循的程序符合本院人体试验委员会所制定的伦理学标准,得到该委员会的批准,分组征得受试对象本人的知情同意,并与之签署临床研究知情同意书。结果①研究组卵巢恶性肿瘤标本中,VEGF121,165表达率明显高于对照组,差异有显著意义(P<0.05),而VEGF189仅在研究组(恶性肿瘤)表达。②VEGF mRNA亚型与卵巢恶性肿瘤临床分期无关(χ2=0.259,P>0.05)。③研究组淋巴结阳性及大网膜转移患者的肿瘤标本VEGF 189阳性表达率明显高于研究组淋巴结阴性及大网膜正常标本,差异有显著意义(P<0.05)。④研究组表达VEGF189者平均微血管密度为20.18±9.00明显高于VEGF189阴性者(16.87±7.31),差异有显著意义(P=0.003)。结论 VEGF 121,165表达与卵巢恶性肿瘤发生发展相关;VEGF 189表达与恶性肿瘤浸润转移有关,组织表达者较不表达者易发生远处转移。因此,VEGF 189可作为预测肿瘤转移潜能的指标。VEGF189与卵巢恶性肿瘤组织血管生成密切相关。Objective To investigate the expression of subtypes of vascular endothelial growth factor （VEGF） mRNA in ovarian neoplasm and its clinical significance. Methods From January 2000 to June 2004, tumor samples from 54 cases （research group） of ovarian malignant tumor were recruited into this study. Among them, ovarian epithelial carcinoma was 41 cases, and non-epithelial carcinoma was 13 （control group）. The expressions of VEGF mRNA subtypes in ovarian malignant tumor and controls were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Meanwhile, an antibody to von willebrand factor （F8） was used to detect tumor microvessel density （MVD）. The study protocol was approved by Beijing General Army Hospital. Informed consent was obtained from all participants. Results （1）The expression positive rates of VEGF 121, VEGF 165 in ovarian malignant were significantly higher than those in benign tumor and normal control （P〈0. 05）; VEGF 189 were only expressed in ovarian malignant. （2）No significantly association were between expressive levels of VEGF mRNA subtypes and clinical stage （X2=0. 259,P〉0.05）. （3）The positive expression rate of VEGF 189 in patients with lymph node metastasis and omentum involvement were significantly higher than those in non-lymph node metastasis and normal omentum （P〈0. 05）. （4）There was a close relationship between VEGF 189 expression and microvessel density in ovarian malignant （P-0. 003）. Conclusion The expression of VEGF 121, 165 were correlated with the carcinogenesis and progress of ovarian malignant. VEGF 189 might be a biomarker for the status of ovarian carcinomas present in invasion and metastasis. VEGF 189 plays an important role in the angiogenesis of ovarian malignant.

关 键 词：血管内皮生长因子 微血管密度 卵巢肿瘤 浸润 转移 

分 类 号：R737.31[医药卫生—肿瘤]