烹调油烟中细颗粒物PM_(2.5)对A-549细胞存活率和IL-10水平的影响  

Effects of PM_(2.5) in cooking oil fume on cell survival and IL-10 levels in A-549 cells

作　　者：冯哲伟[1] 梁春梅[1] 操基玉[1,2] 郭冬梅[1] 王勇[1] 王绩凯[1] 

机构地区：[1]安徽医科大学公共卫生学院,安徽合肥230032 [2]安庆医药高等专科学校,安徽安庆246052

出　　处：《中国工业医学杂志》2011年第2期104-106,共3页Chinese Journal of Industrial Medicine

基　　金：安徽省教育厅自然科学基金(2006KJ311B);安徽省自然科学基金(090413265X)

摘　　要：探讨烹调油烟中大气细颗粒物PM2.5(FPCOF)对人肺癌上皮细胞(A-549细胞)抗炎因子IL-10分泌的影响。分别用不同浓度的FPCOF对细胞进行染毒,于12 h、24 h和36 h后用MTT法测细胞存活率;用酶联免疫吸附法测染毒24 h和36 h的细胞上清中IL-10的浓度。结果 :(1)FPCOF作用于A-549细胞24 h和36 h后,细胞存活率随染毒浓度升高而降低,100μg/ml PM2.5+10 mmol/L NAC组存活率高于100μg/ml PM2.5染毒组。(2)FPCOF对A-549细胞作用24 h后,对抗炎因子IL-10的影响不明显;作用36 h后均可见IL-10水平增高,100μg/ml PM2.5+10mmol/L NAC组IL-10浓度低于100μg/ml PM2.5染毒组。提示FPCOF促进A-549细胞中抗炎因子IL-10分泌,其表达要迟于FPCOF对细胞增殖的抑制作用,抗氧化剂NAC对A-549细胞有一定的保护作用。Objective To explore fine particle in cooking oil fume（FPCOF,PM2.5）on the level of anti-inflammatory factor IL-10 in human lung adenocarcinoma epithelial cell（A-549 cells）.Methods A-549 cells were exposed to different concentrations of FPCOF,for 12 h,24 h and 36 h,respectively,then detecting the cell survival rate with MTT assay and IL-10 level in media supernatant by enzyme linked immunosorbent assay.Results（1）The survival rate of A-549 cells was reduced time-and dose-dependently,but the difference at 12 h among different doses was not significant,meanwhile the rate in 100 μg/ml PM2.5＋10 mmol/L NAC group was significantly higher than that in 100 μg/ml PM2.5 group after 24 h or 36 h exposure.（2）There was no obvious effect on IL-10 after 24 h exposure to FPCOF,the rise of IL-10 level was only seen in all the exposed groups after 36 h exposure to FPCOF;additionally,IL-10 level in 100 μg/ml PM2.5＋10 mmol/L NAC group was significantly lower than that in 100 μg/ml PM2.5 group.Conclusion FPCOF might promote the secretion of anti-inflammatory factor IL-10 in A-549 cells,but its expression was later than the inhibition of cell proliferation caused by FPCOF;and the antioxidant NAC might protect A-549 cells from the adverse effects induced by FPCOF.

关 键 词：烹调油烟 A-549细胞 抗炎因子 白介素-10(IL-10) 

分 类 号：R122.3[医药卫生—环境卫生学]