在多发性骨髓瘤中bcl-1基因重排、细胞周期D_1蛋白高表达及其临床意义  

Bcl-1 Rearrangement and Highcyclin D_1 Protein Expression in Multiple Myeloma and Their Clinical Implication

作　　者：唐泽海[1] 游泳[1] 刘新月[1] 李惠玉[1] 向建平[1] 邹萍[1] 

机构地区：[1]同济医科大学附属协和医院血液科,武汉430022

出　　处：《华中医学杂志》1999年第5期197-198,221,共3页Central China Medical Journal

摘　　要：21例多发性骨髓瘤患者的外周血和骨髓的单个核细胞,同时用免疫组织化学和两步法半筑巢式多聚酶链反应(PCR)技术进行分析,检出5例(24%)细胞周期D_1蛋白高表达,4例(19%)bcl-1基因重排.该4例bcl-1基因重排的患者都高表达细胞周期D_1蛋白.5例细胞周期D_1蛋白高表达的患者都具有较晚的临床分期、骨髓中瘤细胞比率较高、化疗效果差等特点.The rearrangement of bcl-1gene and the expression of cyclin D] protein in peripheral blood and marrow mononuclear cells in 21 multiple myeloma (MM) patients were simultaneously analyzed by using immunohistochemistry method and hemi-nested polymerase chain reaction (PCR). 4/21(19%) MM patients displayed bcl-1 rearrangement by PCR and 5/21 (24%)MM patients showed cyclin D1 overexpression by immunohistochemistry method. All 4 cases of bcl-1 rearrangement had overexpression of cyclin D1 protein, bcl-1 rearrangement and(or)cyclin D1 protein overexpression was a poor prognostic marker for MM patients. Five cases of cyclin D1 protein overexpression were characterized by advanced clinical stage .a highly disseminated tumor,and the refractoriness to standard therapy.

关 键 词：多发性骨髓瘤 bcl-1基因重排 细胞周期D1蛋白 

分 类 号：R733.303[医药卫生—肿瘤]