均匀设计法优化廉价型牛凝乳酶工程菌发酵培养基  

Optimization of Fermentation Medium for Genetically Engineered Chymosin Strain by Uniform Design Methodology

作　　者：袁伟[1,2] 惠丰立[2] 柯涛[2] 程民杰[2] 赵金梅[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]南阳师范学院生命科学与技术学院,河南南阳473061

出　　处：《食品科学》2011年第7期258-261,共4页Food Science

基　　金：河南省科学技术厅基础与前沿技术研究计划项目(102300410146);河南省教育厅自然科学基金项目(2010A180017)

摘　　要：为了获得生产用廉价型牛凝乳酶工程菌发酵培养基,通过单因素试验考察发酵培养基中各组分对产酶的影响。结果显示:葡萄糖、玉米浆、酵母提取物、尿素质量浓度对产酶影响显著。以上述因素作为随机因子,进行均匀设计试验,采用逐步回归方法对试验结果进行分析。结果表明:在葡萄糖45g/L、玉米浆17g/L、酵母提取物6g/L、尿素12g/L的条件下,凝乳酶活性达342.86SU/mL,比优化前提高了1.22倍。所得培养基为重组牛凝乳酶的高效低成本生产提供了参考。In order to obtain a low-cost fermentation medium for recombinant chymosin strain Chy1 in a large scale,the effects of glucose,corn syrup,yeast extract and urea concentration on chymosin production by recombinant chymosin strain Chy1 were explored by single factor tests.The results indicated that the effects of glucose,corn syrup,urea and yeast extract on chymosin production were significant.The optimal fermentation medium was optimized by uniform design tests to be glucose of 45 g/L,corn syrup of 17 g/L,yeast extract of 6 g/L and urea of 12 g/L.Under these optimal conditions,the activity of chymosin can reach up to 342.86 SU/mL,which exhibited a 1.22 fold enhancement when compared with original medium.This optimal fermentation medium provided a basis for massive chymosin production by recombinant chymosin strain Chy1 with high efficiency and low cost.

关 键 词：凝乳酶 工程菌 均匀设计 培养基 

分 类 号：Q949.9[生物学—植物学]