葡萄籽原花青素对顺铂所致人胚肾细胞毒性的拮抗作用  

Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-induced Nephrotoxicity in HEK293 Cell

作　　者：李贞[1] 高丽萍[1] 冷洪涛[1] 张海莲[1] 

机构地区：[1]北京联合大学应用文理学院,生物活性物质与功能食品北京市重点实验室,北京100083

出　　处：《食品科学》2011年第7期315-318,共4页Food Science

基　　金：北京市教委面上项目(KM200911417004)

摘　　要：目的:体外研究葡萄籽原花青素提取物(GSPE)对顺铂(CDDP)所致人胚肾细胞毒性的保护作用,并探讨其可能的机理。方法:体外培养人胚肾细胞(HEK293),四甲基偶氮噻唑蓝(MTT)法观察葡萄籽原花青素、CDDP对HEK293细胞生长的影响及葡萄籽原花青素对CDDP所致细胞毒性的保护作用,硫代巴比妥酸法观察葡萄籽原花青素对CDDP引起脂质过氧化的影响,利用二硫代二硝基苯甲酸与巯基化合物反应,比色定量测定吸光度以观察葡萄籽原花青素对CDDP诱导的谷胱甘肽耗竭的影响。结果:低浓度的GSPE可显著增强HEK293的细胞活性,适宜浓度的GSPE可显著抑制CDDP对HEK293细胞的毒性作用,GSPE可减缓CDDP引起的谷胱甘肽的耗竭。结论:GSPE能抑制CDDP诱发HEK293细胞的细胞毒性,其机理可能与GSPE强抗氧化功能和猝灭自由基的功能有关。Objective：To investigate the protective effect of grape seed proanthocyanidin extract（GSPE） against cisplatin（CDDP）-induced nephrotoxicity in human embryo kidney（HEK） 293 cells and its possible mechanisms.Methods：The effects of GSPE and CDDP on the in vitro growth of HEK 293 cells and the effect of GSPE on CDDP-induced nephrotoxicity were observed by MTT assay.TBARS assay was used to evaluate the effect of GSPE on GSPE-induced lipid peroxidation.The effect of GSPE on CDDP-induced glutathione depletion was examined by colorimetrically determining absorbance resulting from the reaction between DTNB and hydroxyl compounds.Low concentrations of GSPE could remarkably enhance the activity of HEK 293 cells.CDDP-induced nephrotoxicity in these cells was obviously inhibited by appropriate concentrations of GSPE.Additionally,GSPE could also alleviate CDDP-induced glutathione depletion.Conclusion：GSPE has the potential to inhibit CDDP-induced nephrotoxicity in HEK 293 cells,and the possible mechanism is associated with the strong antioxidant function and free radical quenching activity of GSPE.

关 键 词：顺铂 葡萄籽原花青素提取物 肾毒性 HEK293 氧化应激 

分 类 号：Q946.8[生物学—植物学]