食管癌端粒酶活性的研究  

The telomerase activity in human esophageal cancer

作　　者：张登学[1] 张翠萍[1] 顾华丽[1] 唐晓燕[1] 刘海灵[2] 

机构地区：[1]青岛医学院附属医院,266003 [2]中国科学院肿瘤研究所

出　　处：《中华消化杂志》1999年第5期298-300,共3页Chinese Journal of Digestion

摘　　要：目的　探讨食管癌端粒酶活性检测的临床意义。方法　建立并应用ＰＣＲ 技术为基础的端粒重复序列扩增，对３６ 例食管癌及其癌旁组织进行检测。结果　３６ 例食管癌组织中２９ 例端粒酶活性呈阳性，阳性检出率为８０ ．５０ ％ ，癌旁组织中２ 例呈阳性，阳性检出率为６．００％ ；二者比较差异有非常显著性（χ２ ＝ ４１．３０，Ｐ＜０ ．００１）；３６ 例食管癌均为鳞状细胞癌，其中１７ 例伴随淋巴结转移的标本中端粒酶活性均呈阳性，而在１９ 例未伴随淋巴结转移的病人中仅有１２ 例检出端粒酶活性，其活性检出率为６３．１５％ ，二者比较差异有非常显著性（ Ｐ＝ ０ ．００６）。结论　端粒酶活性的检测可作为食管癌诊断的指标之一，同时对判断食管癌的预后具有重要参考价值。Objective To evaluate the role of telomerase activity in human esophageal cancer. Methods Using PCR based telomeric repeat amplification protocol assay, telomerase activity was examined in 36 specimens of esophageal carcinoma (EC) and their adjacent tissues obtained from patients who underwent surgical resections. Results It was found that telomerase activity was positive in 29 of the 36 esophageal carcinoma specimen (80.50%) and in 2 of the 36 adjacent tissue specimen (6.00%) (χ 2=41.30, P <0.001); the positive rates of telomerase activity in 17 EC with lymph node metastasis and in 19 EC without lymph node metastasis were 100% and 63.15%, respectively ( P =0.006). Conclusion It suggests the activity of telomerase might be a diagnostic marker of EC, and might play an important role in prognosis of EC.

关 键 词：食管肿瘤 端粒酶 聚合酶链反应 

分 类 号：R735.102[医药卫生—肿瘤]