下咽癌中P53基因点突变的PCR—SSCP检测  

PCR-SSCP Detection of P^53 gene Mutations in Hypopharyngeal Squamous Cell Carcinoma

作　　者：刘克杰[1] 张信行[1] 郑卫东[1] 王惠忠 张广茹 朱春晓 

机构地区：[1]山东省临沂医学专科学校,276002 [2]山东省淄博侨联医院 [3]山东省临沂市中医院

出　　处：《医学理论与实践》1999年第10期574-575,578,共3页The Journal of Medical Theory and Practice

摘　　要：为探讨下咽癌中P^(53)基因突变的规律与特征,采用PCR—SSCP方法检测40例下咽癌新鲜手术标本组织,分析P^(53)基因突变与下咽癌有关病理指标的关系。40例下咽癌有28例(70％)出现P^53基因第5～8外显子突变。P^(53)基因突变与下咽癌TNM分期、病理分级及淋巴结转移密切相关(P<0.05)。结果显示P^(53)基因突变与下咽癌的生物学行为有关且具有预后价值。To analyse the regularity and characteristics of P53 gene mutation in hypopharyngeal squamous cell carcinoma (HPC). 40 cases of frsh tissues of HPC were detected by PCR-SSCP,and the relationship was studied between P53 gene mutations and the clinicopathological parameters of HPC. Themutations in exon 5-8 of the P53 gene were demonstrated in 28 cases (70% ), It was found that P53 gene mutation was correlated with TNM staging, histopathological grading and regional lymph nodes metastasing, ( P <0. 05). These findings suggest that P53 gene mutations are associated with biological behavior of HPC,and that PCR -SSCP method reveals practical significance for evaluating prognosis of HPC.

关 键 词：下咽癌 P53基因 PCR-SSCP 检测 

分 类 号：R739.63[医药卫生—肿瘤]