光动力学疗法联合5-氟尿嘧啶治疗小鼠移植性实体肿瘤  

Experimental Study of Photodynamic Therapy Combined with Chemotherapy of Subcutaneously Transplanted Hepatomain Mice

作　　者：盛梓松 顾瑛[2] 黄英才[2] 

机构地区：[1]武装警察部队总医院保健办公室,北京市100039 [2]解放军总医院激光科

出　　处：《中国激光医学杂志》1999年第3期148-150,共3页Chinese Journal of Laser Medicine & Surgery

摘　　要：目的　探讨增强光动力学疗法(PDT) 治疗恶性肿瘤疗效的有效途径。方法　80 只昆明种小白鼠于皮下接种小鼠肝癌细胞,待肿瘤长到6 ～8 mm ,随机分为先化疗后PDT、先PDT 后化疗、单纯PDT、单纯化疗和空白对照共5 组,每组16 只。化疗方法为腹腔注射5氟尿嘧啶75 μg/g ;PDT 方法为经尾静脉注射血卟啉单醚20μg/g 后3 h 以能量密度135 J/cm2 的铜蒸气激光照射肿瘤部位。结果　采用不同治疗措施的4 组小鼠瘤体缩小,而空白对照组小鼠瘤体迅速增大,各治疗组与空白对照组比较,差异均有显著意义( P< 0-05) 。各治疗组中以先化疗后PDT 组瘤体缩小尤为显著,与其他3 组比较,差异有显著意义( P< 0 .05) 。结论　PDT 和化疗均有抑瘤效应,两种方法联合运用可提高对小鼠移植瘤的疗效,而先化疗后PDT 治疗的疗效优于先PDT 后化疗。Objective To explore the possibility ofphotodynamictherapy (PDT) combined with chemotherapy (CMT)toimprovethe effectiveness ofcan certreatment. Methods 80 mice with subcutaneously transplanted hepatoma that grew to 6 8 m m were randomly divided into 5 groups: CMT PDT; PDT CMT;PDT;CMTand control. Each group had 16 mice.CMT:5 Fu (75 μg/g) wasinjectedi.p .PDT:Hematoporphyrin monomethyl ether (HMME) (20 μg/g) wasinjectedi.v.and followed bylaserirradiation (wavelength 510 .6 nm ,power density 135 J/cm2) 3 hourslater. Results The size ofthetumorsin 4 groups with differenttreatmentreduced rapidly andthetumorsin controlgroup enlargedrapidly .Astatis tically significant difference wasfound between the experimental groups and the control group ( P< 0 .05) .Inthe experimental groups,thein hibition rate of CMT PDTgroup was significantly higherthan thatofother3 groups.The differences were statistically significant( P< 0 .05) . Conclusions PDTand CMThaveinhibition effect ontumors.The resutlsrevealed that PDTcombined with CMTmight excert better destructive effecton tumors ,and the destructive effect of CMT PDTwas significantly higherthan thatof PDT CMT.

关 键 词：光化学疗法 药物疗法 肿瘤 

分 类 号：R730.57[医药卫生—肿瘤]