改良MTT法在卵巢癌细胞株化疗药物敏感试验中应用的研究  

Chemosensitivity testing of Human ovarian cancer cell lines by the reformed MTT assay

作　　者：黄曼婷[1] 郑建华[1] 孙云桥[1] 于红丽[1] 刘嘉琦[1] 韦淑芹 

机构地区：[1]哈尔滨医科大学附属第一医院妇产科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》1999年第5期372-375,共4页Journal of Harbin Medical University

摘　　要：探讨ＭＴＴ法药敏试验对卵巢癌临床化疗的指导意义。采用改良ＭＴＴ法检测了６ 株卵巢癌细胞对１０ 种化疗药物１０ 种不同浓度的药物敏感性。通过卵巢癌细胞株对化疗药物的量效关系曲线求出药物的ＩＤ５０ 值及ＡＵＣａｔＩＤ５０ 值来判断药效。结果表明，①药敏试验结果与卵巢癌细胞株的病理类型、原代细胞取材部位无肯定关联，但各卵巢癌细胞株对化疗药物的敏感性有显著性差异（Ｐ＜０－００１）；②以ＩＤ５０ 法判定，ＩＦＭ 对５ 株卵巢癌细胞有效，ＣＰＭ、ＡＣＴＤ 对４ 株有效，ＰＭＭＣ、ＶＢＬ对２ 株有效，以ＡＵＣ法判定，ＩＦＭ 对６ 株有效，ＮＫ６１１ 对４ 株有效，ＣＰＭ 对３ 株有效。提示改良ＭＴＴ法药敏试验结果为卵巢癌临床化疗提供了客观依据。To provide objective basis for individualizing chemotherapy of ovarian cancer by the MTT assay as in vitro chemosensitivity test,the reformed MTT assay was adopted for chemosensitivity testing of 6 human ovarian cancer cell lines.Ten chemotherapeutic agents were used in this test.From the dose effect curve of ovarian cancer cell lines to the agents with 10 different concentrations,we obtained the values of ID 50 and AUC at ID50 to evaluate the potency.Results showed that ①Chemosensitivity had no positive relationship with the pathological classifications and primary focus sites.But,for different cell lines,the difference of chemosensitivity was significant ( P <0.001);②Using the assay ID 50 ,5 cell lines were sensitive to IFM,4 cell lines were sensitive to CPM,ACT D and 2 sensitive to P MMC,VBL.Using the assay AUC,6 cell lines were sensitive to IFM,4 sensitive to NK611 and 3 sensitive to CPM.The results suggest that the reformed MTT assay can be used as a in vitro chemosensitivity test which has great significance of directing clinical chemotherapy for patients with ovarian cancer.

关 键 词：卵巢肿瘤 治疗 抗癌药物 敏感试验 

分 类 号：R737.310.5[医药卫生—肿瘤] R979.1[医药卫生—临床医学]