不同氮源对长白山鸡爪苓菌丝体生长及几种胞外酶活性的影响  

Effect of different nitrogen sources medium on growth and several kinds of ectoenzyme activity of Polyporus umbellatus hypha in Changbai Mountain

作　　者：李艳茹[1] 王健[2] 于海茹 许广波[1] 李太元[1] 

机构地区：[1]延边大学农学院,吉林延吉133002 [2]吉林农业大学生命科学学院,吉林长春130118 [3]汪清县特产局,吉林汪清133200

出　　处：《延边大学农学学报》2011年第1期1-5,共5页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金项目(30460084)

摘　　要：研究3种不同氮源(蛋白胨、硝酸钠、硫酸铵)对长白山鸡爪苓菌丝体生长及其胞外蛋白酶、多酚氧化酶及纤维素酶活性的影响.结果表明:3种不同氮源对鸡爪苓菌丝体生长有显著影响,在蛋白胨培养基中菌丝生长速度快、菌丝洁白、菌丝体生物量大;鸡爪苓菌丝体的胞外蛋白酶活性在蛋白胨培养基中达到最高;多酚氧化酶和纤维素酶的酶活性在3种不同培养基中亦表现出很大的差异.Under liquid culture conditions,the effect of three kinds of carbon sources medium on extracellulase,Polyphenol Oxidase and proteinase activity and mycelial growth by Polyporus umbellatus hypha in pure culture was studied.The results showed that effect of different carbon sources medium on mycelial growth of Polyporus umbellatus hypha were significant,in maltose medium the mycelial growth rate was faster than others,the hypha was very white and the biomass was great;the enzyme activity of proteinase was much higher in maltose medium;enzymatic activities of Polyphenol Oxidase and Cellulase are significantly different in different culture mediums.

关 键 词：鸡爪苓菌丝 不同氮源 蛋白酶 纤维素酶 多酚氧化酶 

分 类 号：S182[农业科学—农业基础科学]