应用孕鼠腹腔注射脂多糖合并缺氧方法制作脑瘫动物模型的实验研究  

Establishment of rat cerebral palsy model by intraperitoneal injection of lipopolysaccharide combined with hypoxia in pregnant rat

作　　者：高峰[1] 杨小朋 陈刚 

机构地区：[1]石河子大学医学院外科系,新疆石河子832002 [2]新疆自治区人民医院神经外科,新疆乌鲁木齐830000

出　　处：《临床和实验医学杂志》2011年第7期489-491,共3页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金(No.30960393);新疆维吾尔自治区科技攻关和重点科技课题资助项目[No.200633128(2)];\新疆维吾尔自治区卫生厅青年科技人才专项科研基金(No.2007Y26)

摘　　要：目的探讨孕鼠腹腔注射脂多糖宫内感染合并缺氧致幼鼠脑瘫的模型制作及评价方法。方法实验分为3组:脂多糖缺氧组,受孕17 d的健康Wistar孕鼠脂多糖腹腔注射,12 h后置于缺氧环境中2.5 h,4 h后再次腹腔注射脂多糖;颈动脉结扎缺氧组,7 d龄Wistar幼鼠结扎右侧颈动脉结合缺氧环境2 h;生理盐水对照组,受孕17 d健康Wistar孕鼠腹腔注射同剂量的生理盐水。于生后4周采用足印步态重复间距分析试验、平衡木试验,后取鼠脑组织行病理学检测。结果与对照组相比,脂多糖缺氧组和颈动脉结扎缺氧组前后肢足印重复性差,表现为足印间距增大,且间距不稳定(P<0.05);脂多糖缺氧组与颈动脉结扎缺氧组之间在足印重复间距、平衡术通过时间方面无显著差异(P>0.05);脂多糖缺氧组组织病理学示:患侧脑室旁正常组织结构破坏,细胞排列紊乱,局部囊性变和炎性细胞聚积。结论应用孕鼠腹腔注射脂多糖合并缺氧的方法制作脑瘫模型稳定、可靠,与单侧颈动脉结扎缺氧模型相比具有微创、简单易行、更逼真地模拟人类围产期感染、缺血缺缺氧致胎儿脑部损伤的优点。Objective To establish and evaluate the rat cerebral palsy model by intraperitoneal injection of lipopolysaccharide（LPS） combined with hypoxia in pregnant rat.Methods The experimental rats were divided into LPS-hypoxia group,hypoxia group and control group.In LPS-hypoxia group,the pregnant rats were intraperitoneally injected with LPS on gestational day 17,then exposed to hypoxic environment in 12 h for 2.5 h and followed by the second LPS injection in 4 h.In hypoxia group,the rats had the left carotid artery ligated followed by hypoxia for 2 h on postnatal day 7.In control group,the pregnant rats were intraperitoneally injected with same amount of normal saline on gestational day 17.Footprints gait repeat spacing analysis,balance beam test and pathological examinations were performed in postnatal 4 wk rats.Results Compared with control group,poor footprints repeatability,increased footprints spacing and the instable spacing（P0.05） were found in LPS-hypoxia group and hypoxia group.The footprints gait repeat spacing,balance beam test were no significant difference LPS-hypoxia group and hypoxia group（P0.05）.Pathological examination revealed structural damage,local cystic degeneration and inflammatory cells accumulation in the tissue adjacent to cerebral ventricle.Conclusion The rat cerebral palsy model by intraperitoneal injection of LPS combined with hypoxia in pregnant rat is of advantages of easy to operate,minimally invasive and more closely simulate fetal brain damage caused by perinatal infection,hypoxia and ischemia in comparison with the model by unilateral carotid artery ligation induced hypoxia.

关 键 词：脑瘫 脂多糖 炎症 缺血缺氧 动物模型 

分 类 号：R-332[医药卫生]