急性白血病免疫表型分析的临床应用  

The clinical application of immunophenotyping in leukemia

作　　者：徐翀[1] 沈立松[1] 胡芳华[1] 王淑香[1] 沈霞[1] 

机构地区：[1]上海第二医科大学附属新华医院,200092

出　　处：《上海医学检验杂志》1999年第5期299-301,共3页Shanghai Journal of Medical Laboratory Sciences

摘　　要：用流式细胞术对６０ 例初诊或未缓解的急性白血病患者进行免疫表型检测。ＡＭＬ患者均表达髓系抗原，部分病例同时伴有淋巴系抗原的表达。ＡＬＬ患者均表达淋巴系抗原，但２８．６％ 病例同时表达髓系抗原（ＣＤ１３或ＣＤ３３ 。）。２ 例急性髓系与淋系双表型杂合性白血病同时表达２个以上淋巴和髓系抗原标志。１ 例急性Ｔ系与Ｂ系双克隆杂合性白血病两群白血病细胞分别表达Ｔ、Ｂ系抗原。１ 例形态学检查为ＡＭＬ，但表达全部３ 个Ｂ淋巴抗原和ＣＤ１３ ，而诊断为伴髓系抗原表达的急性淋巴细胞性白血病。结果表明，结合免疫表型分析与细胞形态学检验能为急性白血病的诊断提供更可靠和更有价值的分型依据。patients with primary or partial or not remission leukemia were determined with flow cytometry to obtain valid diagnosis.All myeloid leukemia patients expressed myeloid markers,but some cases also expressed lymphoid lineage antigens.Lymphoid antigen expression was found in all acute lymphoblastic leukemia,but 28 6%(10/35)of these patients also expressed myeloid markers CD 13 or CD 33 .Two cases are myeloid and lymphoid hybrid type leukemia that expressed more than 2 markers of both lymphoid and myeloid origins.One case was T and B hybrid type whose leukemic cells divided into two distinct populations expressing T or B antigens simultaneously.One case displayed morphological characteristics of myeloid leukemia,but immunophenotyping results revealed that this was actually acute lymphoblastic leukemia with myeloid antigen expression,since all three B antigens and CD 13 were strongly expressed.Our data indicate that immunophenotyping,combined with morphology can provide more reliable and valuable evidence in the diagnosis of leukemia.

关 键 词：白血病 流式细胞 免疫表型 急性 

分 类 号：R733.71[医药卫生—肿瘤]