气相色谱法同时测定吐温80中7种脂肪酸亲油基的含量  

GC determination content of seven fatty acid hydrophobic group in Tween-80

作　　者：丛英[1] 苏柘僮[1] 邹文铨[2] 杨明[1,3] 马鸿雁[1] 张海燕[3] 

机构地区：[1]成都中医药大学,成都610075 [2]四川大学,成都610064 [3]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《药物分析杂志》2011年第4期651-654,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：中医药行业科研专项项目(200708006)

摘　　要：目的:建立用GC测定吐温80中亲油基含量的方法,为吐温80使用适宜性研究奠定基础。方法:采用GC法,选用DB-1毛细管色谱柱,柱温185℃,进样口温度250℃,检测器温度250℃。结果:吐温80中7种亲油基衍生的脂肪酸甲酯(油酸甲酯、亚油酸甲酯、硬脂酸甲酯、肉豆蔻酸甲酯、棕榈油酸甲酯、棕榈酸甲酯、亚麻酸甲酯)在测定范围(1.8～35.5 mg;0.4～2.1mg;0.5～4.1 mg;0.2～1.2 mg;0.6～2.9 mg;0.5～2.7 mg;0.6～3.0 mg)内有良好线性关系;油酸甲酯的平均回收率为100.7%。结论:本方法简单,稳定易行,可控性强,重复性好,可用于控制吐温80的质量。Objective： An GC method for determination the quantitative analysis of hydrophobic group in Tween-80 was developed to provide the theoretical basis for Tween-80.Methods：DB-1 capillary column was used,column temperature was 185 ℃,injection port temperature of GC was set at 250 ℃,detector temperature was set at 250 ℃.Results：Methyl oleate,methyl linoleate,methyl stearate,methyl myristate,methyl palmitoleate,methyl palmitate,methyl linolenate assumed fine linear relationship at the sample size of 1.8-35.5 mg,0.4-2.1 mg,0.5-4.1 mg,0.2-1.2 mg,0.6-2.9 mg,0.5-2.7 mg,0.6-3.0 mg,respectively;The average recoveries of the method was 100.7%.Conclusion：The method is reliable,and can be used for quality control of Tween-80.

关 键 词：吐温80 7种脂肪酸 亲油基 气相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]