UPLC法测定通塞脉微丸中绿原酸、阿魏酸、甘草苷、肉桂酸的含量  

UPLC determination of chlorogenic acid,ferulic Acid, liquiritin and cinnamic acid in Tongsaimai pellets

作　　者：王皓[1] 狄留庆[1] 文红梅[1] 赵晓莉[1] 陶金华[1] 汪海鸿[1] 

机构地区：[1]南京中医药大学,南京210029

出　　处：《药物分析杂志》2011年第4期655-658,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技部863计划(2003AA2Z3247);国家自然基金项目(30873401);江苏省自然基金项目(BK2005150);江苏省"青蓝工程"科技创新团队支持计划

摘　　要：目的:建立同时测定通塞脉微丸中绿原酸、阿魏酸、甘草苷、肉桂酸4种成分的超高效液相色谱(UPLC)分析方法。方法:采用Waters AcquityUPLC系统,AcquityUPLC BEH C18(50 mm×2.1 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸水为流动相,梯度洗脱,双波长检测(λ1=276 nm、λ2=327 nm),柱温35℃。结果:绿原酸、阿魏酸、甘草苷、肉桂酸在16 min内即达到基线分离,在测定的浓度范围内线性关系良好(r≥0.9997),各成分的平均回收率在95.1%～98.2%,RSD<4.0%。结论:UPLC分离效果及重复性好,且快速、简便,可作为通塞脉微丸的质量控制方法。Objective：To establish a ultra performance liquid chromatography（UPLC） method for simultaneous determination of 4 components—chlorogenic acid,ferulic acid,liquiritin and cinnamic acid in Tongsaimai pellets.Methods：The analyses were performed on a Waters AcquityUPLC system equipped with a binary solventmanager,a samplermanager,a column compartment and a PDA detector,connected to Waters Empower 2 software.An AcquityUPLC BEH C18 column（50 mm×2.1 mm,1.7 μm）was used for all analyses.The investigated compounds were separated with gradient mobile phase consisting of acetomitrile-0.1% formic acid.Chlorogenic acid,ferulic acid,liquiritin and cinnamic acid were determined by dual wavelength of 276 nm（λ1） and 327 nm（λ2）,and column temperature 35 ℃.Results：Four components could be well separated at the baseline in 16 min.The average recovery was 95.1% to 98.2% with RSD4.0%.Conclusion：The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of Tongsaimai pellets.

关 键 词：通塞脉微丸 绿原酸 阿魏酸 甘草苷 肉桂酸 超高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]