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作 者:刘春莹[1] 宋建国[2] 李鹏飞[1] 鱼红闪[1] 金凤燮[1]
机构地区:[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连工业大学化工与材料学院,辽宁大连116034
出 处:《大连工业大学学报》2011年第2期79-82,共4页Journal of Dalian Polytechnic University
基 金:国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(2008T0082009T007LT2010009)
摘 要:研究了市销人参、西洋参和三七参根部中总皂苷的含量及组成。采用正丁醇萃取法提取总皂苷发现:总皂苷在人参主根、须根中的质量分数分别为4.0%、6.1%;在西洋参主根、须根中的质量分数分别为4.1%、6.3%;在三七参主根、须根中的质量分数分别为8.2%、1.7%。人参、西洋参须根中总皂苷的质量分数均高于主根,是其1.5倍;而三七参主根中总皂苷的质量分数高于须根,是其3倍。TLC和HPLC检测结果表明,人参中各种皂苷的质量分数差别不大,主根、须根的w(PPD)/w(PPT)分别为37∶58、66∶33,人参中含有特征皂苷Rf,其质量分数为5%~8%,人参中还含有微量R1。西洋参中Re、Rb1含量较高,占总皂苷的40%~50%,主根、须根的w(PPD)/w(PPT)分别为50∶43、58∶39,西洋参中不含有R1和Rf皂苷。三七参中Rg1、Rb1的质量分数较高,占总皂苷的70%,其主根、须根总皂苷组成相似,三七参中含有特征皂苷R1,其质量分数为9%~11%。The component of ginsenosides in different ginseng was investigated.The content of total ginsenosides extracted by butanol from root and fiber was 4.0% and 6.1% in ginseng,4.1% and 6.3% in gen-seng and 8.2% and 1.7% in notoginseng.The ginsenoside in fiber is higher than that in root for ginseng and gen-seng,but ginsenoside in root is higher than that in fiber for notoginseng.When detected by TLC and HPLC,the value of w(PPD)/w(PPT) were 48∶52 and 70∶30 in the root and fiber respectively and the content of Rf was 5%-8%.Ginseng also contained R1.The higher content of gen-seng is Re and Rb1 which is about 40%-50% in total ginsenoside.The value of w(PPD)/w(PPT) is 57∶43 and 61∶39 in the root and fiber respectively.R1 and Rf can not detected in gen-seng.The higher content of notoginseng is Rg1 and Rb1 with 70% in the total ginsenoside.The composition of total ginsenoside in the root is similar to that in fiber of notoginseng.The characteristic ginsenoside in notoginseng is R1,content of which is 9%-11%.
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