Anti-tumor Immune Response Mediated by Newcastle Disease Virus HN Gene  

作　　者：PENG Li-ping LI Xiao SUN Li-li WEN Zhong-mei LIU Yan GAO Peng HUANG Hai-yan PIAO Bing-guo JIN Jing J1N Ning-yi 

机构地区：[1]Department of Respiratory Medicine, the First Hospital of Jilin University, Changchun 130021, P R. China [2]Genetic Engineering Laboratory of PLA, the Eleventh Institute of Academy of Military Medical Sciences of PLA, Changchun 130062, P R. China [3]Head and Neck Surgery, the Tumor Hospital of Jilin Province, Changchun 130012, P R. China [4]Department of Gastroenterology, First Hospital of Jilin University, Changchun 130021, P R. China [5]Department of Hematology and Oncology, People＇s Hospital of Jilin Province, Changchun 130021, P R. China [6]College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, P R. China

出　　处：《Chemical Research in Chinese Universities》2011年第2期282-286,共5页高等学校化学研究（英文版）

基　　金：Supported by National High-Tech Research and Development Program of China(No.2007AA021004);the National Basic Research Program of China(No.2005CB523005);the National Natural Science Foundation of China(No.30771609);the National Science and Technology Major Project of China(Nos.2008ZX10004-015; 2009ZX08006-002B)

摘　　要：Hemagglutinin-neuramidinase（HN） is one of the most important surface structure proteins of the Newcastle disease virus（NDV）. HN not only mediates receptor recognition but also possesses neuraminidase（NA） activity, which gives it the ability to cleave a component of those receptors, NAcneu. Previous studies have demonstrated that HN has interesting anti-neoplastic and immune-stimulating properties in mammalian species, including humans. To explore the application of the HN gene in cancer gene therapy, we constructed a Lewis lung carcinoma（LLC） solid tumor model using C57BL/6 mice. Mice were injected intratumorally with the recombinant adenovirus expressing HN gene（Ad-HN）, and the effect of HN was explored by natural killer cell activity assay, cytotoxic lymphocyte activity assay, T cell subtype evaluation, and Th1/Th2 cytokines analysis. The results demonstrate that HN not only can elicit clonal expansion of both CD4＋ and CD8＋ T cell populations and cytotoxic T lymphocyte（CTL） and killer cell response, but also skews the immune response toward Th1. Thus, vaccination with Ad-HN may be a potential strategy for cancer gene therapy.Hemagglutinin-neuramidinase（HN） is one of the most important surface structure proteins of the Newcastle disease virus（NDV）. HN not only mediates receptor recognition but also possesses neuraminidase（NA） activity, which gives it the ability to cleave a component of those receptors, NAcneu. Previous studies have demonstrated that HN has interesting anti-neoplastic and immune-stimulating properties in mammalian species, including humans. To explore the application of the HN gene in cancer gene therapy, we constructed a Lewis lung carcinoma（LLC） solid tumor model using C57BL/6 mice. Mice were injected intratumorally with the recombinant adenovirus expressing HN gene（Ad-HN）, and the effect of HN was explored by natural killer cell activity assay, cytotoxic lymphocyte activity assay, T cell subtype evaluation, and Th1/Th2 cytokines analysis. The results demonstrate that HN not only can elicit clonal expansion of both CD4＋ and CD8＋ T cell populations and cytotoxic T lymphocyte（CTL） and killer cell response, but also skews the immune response toward Th1. Thus, vaccination with Ad-HN may be a potential strategy for cancer gene therapy.

关 键 词：HN gene Recombined adenovirus CYTOKINE Cell immunity Anti-tumor activity 

分 类 号：S852.4[农业科学—基础兽医学] S852.659.5[农业科学—兽医学]