Caspase-3基因在凋亡HL-60细胞及肿瘤细胞中的表达  

Expression of caspase 3 gene in apoptotic HL 60 cell and different human tumor cell lines\

作　　者：李小明[1] 童新[2] 宋天保[1] 孙志贤[2] 

机构地区：[1]西安医科大学组织胚胎学教研室,710061 [2]北京放射医学研究所

出　　处：《中华放射医学与防护杂志》1999年第5期297-299,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：目的　明确ｃａｓｐａｓｅ３ 基因在凋亡前后ＨＬ６０ 细胞及不同肿瘤细胞系中的表达特性。方法　用Ｎｏｒｔｈｅｒｎ 杂交技术检测γ射线诱发的凋亡前后ＨＬ６０ 细胞和６ 种肿瘤细胞中ｃａｓｐａｓｅ３ 的ｍＲＮＡ。结果　ｃａｓｐａｓｅ３ 基因在白血病细胞株ＨＬ６０ 、ＣＥＭ 和Ｋ５６２ 细胞及视母细胞瘤细胞ＳＨＳＹ５Ｙ 中表达高于宫颈癌细胞ＨｅＬａ 及乳腺癌细胞ＭＣＦ７ ；在凋亡ＨＬ６０ 细胞表达高于未照射对照ＨＬ６０ 细胞。结论　ｃａｓｐａｓｅ３ 表达水平与细胞寿命、对辐射敏感性及细胞凋亡的发生有关。Objective\ To research the expression of caspase 3 gene in the apoptotic and the control HL 60 cells and in the different human tumor cell lines. Methods Caspase 3 mRNA in the control and γ radiation induced apoptotic HL 60 cells,and in the 6 types of human tumor cell lines,was analysed by Northern blot. Results The caspase 3 gene transcript was more highly expressed in leukemia cells HL 60,CEM,K562 and neuroblastoma SH SY5Y than in cervical adenocarcinoma HeLa and breast carcinoma MCF7,and more highly in the radiation induced apoptotic HL 60 than in the control HL 60 cells. Conclusion The high level of expression of caspase 3 may aid the efforts to understand the tumor cell sensitivity to radiation,apoptosis and its inherent ability to survive.\;

关 键 词：Γ射线 肿瘤细胞凋亡 辐射诱导 CASPASE-3 

分 类 号：R730.55[医药卫生—肿瘤]