γ-刀不同剂量一次单侧照射大鼠尾壳核后前脑星形胶质细胞的变化  

Changes of astrocytes in rat forebrain after unilateral irradiation of nucleus caudate putamen with different single doses of gamma knife

作　　者：杨婷[1] 饶志仁[1] 吴声伶[2] 吴鸿勋[2] 鞠躬[1] 

机构地区：[1]第四军医大学神经科学研究所,西安710032 [2]广东微侵袭神经外科治疗中心

出　　处：《中华放射医学与防护杂志》1999年第5期316-318,共3页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的　作者研究γ刀不同剂量定位照射大鼠脑尾壳核中部后前脑内星形细胞（ＡＳ） 形态和数量的变化以探讨γ刀的放射生物效应，为临床防护提供依据。方法　应用免疫组织化学ＡＢＣ法以ＡＳ的中型丝主要构成成分———胶质细胞原纤维酸性蛋白（ＧＦＡＰ）作为免疫染色标志观察照射后９０ 天ＧＦＡＰ的表达及变化情况。结果　①吸收剂量１０～３０ Ｇｙ 时，ＡＳ在靶区内大量增生，细胞肥大和变性，与对侧形成明显对比；４０ ～６０ Ｇｙ时照射靶区ＡＳ肥大和变性增多；７０ ～１００ Ｇｙ 时，靶区出现边界明确的坏死区，离坏死区越近，细胞变性越明显；靶区和隔区出现明显的水肿，细胞胞体肥大明显。②从低剂量到高剂量，血管扩张变形渐趋严重，８０ Ｇｙ时，血管内膜出现凹凸不平。③在远离照射部位的脑区，双侧反应差别较小。结论　低剂量伽玛刀局部照射，９０ 天时可通过特异性抗体检测出ＡＳ的数量和形态改变；靶区的损伤程度和剂量成正相关。Objective To investigate variations in morphology and number of astrocytes in the rat forebrain after the caudate putamen nucleus was irradiated by differant single doses of gamma knife from 10Gy to 100Gy. Methods Immunohistochemical ABC method was used to stain glia fibrillary acidic protein(GFAP). Results Firstly,at dosage of 10-30Gy,the number of astrocytes was increased remarkably in the target area and the non target areas displayed much less reactive cells;and when irradiated with 40-60Gy,the brain showed more hypertrophic and degenerated astrocytes in the target area;whereas at dosage of 70-100Gy,necrotic hole began to appear and became bigger with an increasing dosage.The nucleus caudate putamen and septal area became swollen and blood vessels dilated,which tended to increase with increasing dosage.Lastly,in areas not directly irradiated by gamma knife,the ipsilateral and contralateral sides showed a less difference in GFAP expression in the forebrain. Conclusion Degree of damage in target area is positively relative to the dosage.

关 键 词：Γ刀 尾壳核 GFAP 星形胶质细胞 颅内肿瘤 

分 类 号：R730.55[医药卫生—肿瘤] R739.410.5[医药卫生—临床医学]