鼻咽癌的放射敏感性预测初探  

Combined detection of radiosensitivity of nasopharygeal carcinoma \

作　　者：史剑慧[1] 程文英[1] 邵松生[1] 冯嘉林[1] 张有望[2] 

机构地区：[1]上海医科大学放射医学研究所,200032 [2]上海医科大学,200032

出　　处：《中华放射医学与防护杂志》1999年第5期347-349,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家八五攻关分题基金

摘　　要：目的　探索原位缺口平移、早熟凝集染色体分析和胞质分裂阻断微核法等技术成为判断鼻咽癌放射敏感性预测指标的可能性。方法　以原位缺口平移法研究了鼻咽癌细胞株ＣＮＥ、ＣＮＥ－ ２Ｚ细胞对γ射线的放射效应；以早熟凝集染色体分析技术和胞质分裂阻断微核法研究了鼻咽癌活检组织的放射敏感性。结果　在０ ～８ Ｇｙ 之间，ＣＮＥ 及ＣＮＥ－ ２Ｚ 的脱氧三磷酸核苷（ｄＮＴＰ） 掺入率与照射剂量间有良好的剂量效应关系，来源于低分化肿瘤的ＣＮＥ－ ２Ｚ细胞株的放射敏感性高于ＣＮＥ；３２ 例鼻咽活检标本进行早熟凝集染色体分析，其早熟凝集染色体周期分布与鼻咽癌患者放疗疗效有明显关系，临床上对放疗呈高敏的患者，晚Ｇ１ 细胞比例显著升高；胞质分裂阻断微核法提示２０ 例患者照射后微核形成有显著个体差异，其中低反应者２ 例有复发倾向。结论　实验结果提示上述３ 项指标可望成为综合判断鼻咽癌放射敏感性的重要参数。Objective\ Comparisons of radiosensitivity in cells derived from different individual or pathologic types of nasopharygeal carcinoma were carried out to evaluate the combined detection of radiosensitivity of nasopharygeal carcinoma. Methods The radiosensitivity of nasophargeal carcinoma cell lines CNE and CNE\|2Z which had been treated with γ\|rays were detected by in situ nick translation(ISNT).The premature chromosome condensation(PCC) technique and cytokinesis\|block micronucleus(CBMN) method were used to study radiation\|induced cytogenetic damage in cell derived from biopsy samples of 52 patients with nasopharygeal carcinoma. Results The radiosensitivity was lower for CNE which was highly differentiated cancerous cell line than that of CNE\|2Z whose differentiation degree was poorer.When the cell lines were exposed to from 1 to 8 Gy of γ\|rays,the incorporation rate of dNTP of the two cell lines increased linearly with increasing dosage.The sutdy of PCC assay in biopsy samples from 32 patients with nasopharygeal carcinoma suggested that when the cells were in late G\-1 phase and had higher survival rate after irradiation,they were more sensitive than those in other phases.The results of CBMN study in 20 patients also exhibited that the lower micronucleus rate,the easier recurrence of cancer. Conclusion The combined detection of radiosensitivity in cells of nasopharygeal carcinoma may provide potential tool for predicting radiosensitivity of carcerous tissues treated with γ\|rays.\;

关 键 词：鼻咽癌 放射敏感性 预测 放射疗法 

分 类 号：R739.630.5[医药卫生—肿瘤]