乳腺癌细针穿刺标本中端粒酶检测的诊断意义  

Significance of telomerase activity detection by fine needle aspiration in patients with breast cancer

作　　者：金顺钱[1] 张伟[1] 滕茂芳[1] 张智慧[1] 刘毅[1] 李茉[1] 曲平[1] 王淑珍[1] 金玉生[1] 王洪平[1] 潘秦镜[1] 刘树范[1] 

机构地区：[1]中国医学科学院

出　　处：《中华病理学杂志》1999年第5期334-336,共3页Chinese Journal of Pathology

摘　　要：目的　检测乳腺癌细针穿刺细胞标本中的端粒酶，探讨其在乳腺癌辅助诊断中的临床意义。方法　采用聚合酶链反应端粒末端重复序列技术检测９９ 例乳腺细针穿刺标本（８３ 例乳腺癌、１２ 例乳腺良性病变和４ 例乳腺炎）中的端粒酶。结果　８３ 例经组织病理学诊断的乳腺癌标本中，６９例细针穿刺阳性标本中６１ 例端粒酶阳性；７ 例细针穿刺可疑阳性标本中５ 例端粒酶阳性，７ 例细针穿刺阴性标本中４ 例端粒酶阳性，端粒酶敏感性为８４．３％ （７０／８３）；端粒酶活性检测和细胞学诊断总符合率为７７．１ ％（６４／８３） 。１２ 例乳腺良性病变标本有４ 例端粒酶阳性，４ 例乳腺炎标本均为阴性。二种方法同时应用可使术前乳腺癌确诊由原来的８３．１％ （６９／８３ 例） 提高到９３．９％ （７８／８３ 例）。结论　乳腺细针穿刺标本端粒酶检测可提高单纯细胞学检查的敏感性、特异性，在乳腺肿物切除术前为临床治疗提供依据。Objective To study the telomerase activity in samples of breast fine needle aspiration specimens and to investigate its potential clinical implications. Methods Ninety nine fine needle aspiration specimens from 83 patients with breast cancers, 12 with benign lesions and 4 with breast inflammation,were studied for the presence of telomerase activity by a PCR based telomere repeat amplification protocol (PCR TRAP). Results Among 83 fine needle aspiration specimens from patients with breast cancer, telomerase activity was detected in 61 of 69 cytologic positive specimens, 5 of 7 cytologic suspicious specimens and 4 of 7 cytologic negative specimens. Sensitivity of telomerase activity detection was 84.3% (70/83); the coincidence rate between telomerase detection and cytologic confirmation was 77.1%(64/83). Of 12 specimens from patients with benign breast lesions, 4 were positive for telomerase activity, but 4 specimens from patients with breast inflammation were all negative. The Combined use of cytological and telomerase detection can improve the detection rates from 83.1%(69/83) to 93.9%(78/83). Conclusion Detection of telomerase in breast tissue samples by fine needle aspiration is a sensitive and specific method, which implies that it would be helpful in preoperative cytological diagnosis of breast cancer.

关 键 词：乳腺肿瘤 端粒酶 聚合酶链反应 细胞学 诊断 

分 类 号：R737.904[医药卫生—肿瘤]