复智胶囊对血管性痴呆大鼠炎性因子、胆碱能系统的时效性影响  

Effect of Fuzhi Capsule on Inflammation Factors and Acetylcholine System in Rats with Vascular Dementia

作　　者：王保奇[1] 程传浩[1] 马云枝[2] 

机构地区：[1]河南中医学院,郑州450008 [2]河南中医学院第一附属医院,郑州450003

出　　处：《中国实验方剂学杂志》2011年第8期176-179,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关项目(0224630073)

摘　　要：目的:观察复智胶囊对血管性痴呆(vascular dementia,VD)大鼠学习记忆及不同时段海马胆碱乙酰转移酶(aholine acetyl transferase,CHAT)表达,乙酰胆碱酯酶(acetylcholinesterase,AchE)活性,转化生长因子α(transformation growthfactor,TNF-α),白细胞介素6(interleukin,IL-6)的影响。方法:健康Ⅱ级Wistar雄性大鼠110只,采用4血管闭塞法建立急性缺血再灌注VD模型。将造模成功的60只动物按体质量排序,按随机数字表法随机分为模型对照组及治疗组,每组30只动物,假手术组动物10只。治疗组每天给复智胶囊4.5 g·kg-1 ig。模型对照组、假手术组每天生理盐水ig。安理申对照组每天给予安理申(加0.5%羧甲基纤维素液研磨配制)0.05 mg·g-1ig。在第3,7,28天,3个时段随机从治疗组、模型组各抽取10只检验其主动回避反应(active avoid reaction,AAR)习得率、血清TNF-α,IL-6含量及海马CHAT表达、AchE活性。结果:缺血再灌注3d即可形成海马胆碱能神经元的不可逆性损伤,复智胶囊在早期可以减轻该损伤过程,但在疗程内对已经造成的胆碱能神经损伤却无显著的改善作用。海马AchE含量在3 d时显著升高,此后保持在低水平状态,复智胶囊治疗后第7,28天时出现下降并接近假手术组。复智胶囊显著降低IL-6在缺血再灌注早期的表达。TNF-ɑ在炎症反应的早期和晚期均有异常表达;复智胶囊治疗后较模型组同时间点显著下降。结论:复智胶囊能确切改善VD大鼠的学习记忆能力;其机制与减轻缺血炎性反应对神经元的危害、上调海马胆碱能系统低水平状态有关。Objective：To observe the effect of Fuzhi capsule on the ability of learning and memory and the hippocampus Ach E activity and CHAT content and the content of IL-6,TNF-ɑ in serum in rats with vascular dementia.Method：The rat model was established by blocking bilateral common carotid arteries permanently,and the model rats were randomly classified into two groups,i.e.Fuzhi Capsule group（4.5 g.kg-1,ig） and model group.Then（3 d,7 d and 28 d after treatment） active avoid reaction（AAR）rate,hippocampus Ach E activity and CHAT content and the content of IL-6,TNF-ɑ in serum were observed.Such factors were detected as hippocampus Ach E activity and CHAT content in serum after treatment.Result：Cholinergic neurons in the hippocampus were damaged irreversibly after 3 d of ischemia-reperfusion.The Fuzhi Capsule could reduce the damage process in earlystage but did not have significant improvement on the damaged cholinergic nerve in the course of treatment.The hippocampus Ach E content was increased significantly after 3 d,then at a low level.Then Fuzhi Capsule increased it in 7 d and 28 d by comparison with the sham operated group,decreased the expression of IL-6 in early ischemia-reperfusion obviously.The expression of TNF-ɑ were abnormal in the early and late inflammation stage.Then treatment of Fuzhi Capsule decreased it obviously comparison with that of the sham operated group.Conclusion：Fuzhi Capsule can improve the ability of learning and memory.This indicates that Fuzhi capsule may mitigate the injury of inflammation and regulate the low level of acetylcholine in hippocampus.

关 键 词：复智胶囊 血管性痴呆 炎性因子 胆碱能系统 

分 类 号：R285.5[医药卫生—中药学]