太湖沉积物中氮循环菌的微生态  

Research on the micro-ecology of nitrogen cycling bacteria in sediments of Lake Taihu

作　　者：岳冬梅[1] 田梦[1] 宋炜[1] 肖琳[1] 杨柳燕[1] 

机构地区：[1]污染控制与资源化国家重点实验室南京大学环境学院,江苏南京210093

出　　处：《微生物学通报》2011年第4期555-560,共6页Microbiology China

基　　金：国家自然科学基金项目(No.30770414);国家973计划项目(No.2008CB418003)

摘　　要：采用荧光原位杂交(FISH)技术,对梅梁湾和贡湖湾湖区沉积物中微生物的主要种群,及氮循环功能微生物的数量和分布进行了研究,发现随着深度增加,细菌和古菌数量均逐渐减少,但古菌在总菌数中所占比例有所增加;梅梁湾沉积物中氨氧化细菌及亚硝酸氧化细菌的数量均高于贡湖湾,随深度增加,两者数量均逐渐减少,但在贡湖湾其占总菌数的比例高于梅梁湾,表明水生植物可能对沉积物中微生物组成及氮循环有重要影响。泉古菌在太湖沉积物中普遍存在且数量高于氨氧化细菌,说明其在淡水湖泊中对氮循环可能有重要作用。The amount and distribution of nitrogen cycling bacteria in sediments of Meiliang Bay and Gonghu Bay of Lake Taihu were studied by FISH analysis.The amount of archaea decreased along the sediment depth,however,its ratio to total microorganisms increased.The amount of Ammonia oxidiz-ing bacteria（AOB） and nitrite oxidizing bacteria（NOB） in Meiliang Bay was higher than that in Gonghu Bay although the amount of both bacteria decreased along the sediment depth.Our results in-dicated that the existence of aquatic macrophyte in Gonghu Bay might affiliate transformation of nitro-gen.Crenarchaeota was universally detected in surface sediments and overnumbered AOB,which indi-cated that it might play an important role in nitrogen cycling.

关 键 词：沉积物 氮循环 原位荧光杂交(FISH) 泉古菌 

分 类 号：X524[环境科学与工程—环境工程]