酶法转化糖苷型沙棘叶黄酮生成苷元工艺优化  

Optimization of Enzymatic Conversion of Flavonoids Extracted from Sea Buckthorn Leaves

作　　者：冯淑环[1] 殷丽君[1] 袁蕊[1] 韭泽悟 李刚 程永强[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]日本国际农林水产业研究中心 [3]青海康普生物科技股份有限公司,西宁810003

出　　处：《农业机械学报》2011年第4期127-132,138,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家自然科学基金资助项目(30972286);"十一五"国家科技支撑计划资助项目(2007BAD83B03)

摘　　要：为提高聚半乳糖醛酸酶(Pectinex Ultra SP-L)转化沙棘叶黄酮苷元得率,在单因素分析的基础上进行响应面试验。选择酶解时间、酶解温度和pH值为自变量,转化得到的黄酮苷元总质量为响应值。结果显示,酶解时间为显著影响因素,酶解温度和pH值影响不显著。确定了酶解转化的最佳条件为:酶解温度50.8℃,酶解pH值5.16,酶解时间2.5h,在此条件下15mg沙棘叶提取物得到黄酮苷元0.1801mg,转化率为62%。In order to increase the yield of sea buckthorn leaves flavonoid aglycones converted from flavonoid glycosides by the enzyme-Pectinex Ultra SP-L,on the basis of single-factor test,response surface analysis method was used.Hydrolysis time,hydrolysis temperature and pH value were chosen to be independent variables.The value of flavonoid aglycones quality was regarded as the response value.The results showed that the hydrolysis time was a significant effect factor and hydrolysis temperature and pH value had no significant impact.The optimum conditions for enzymatic conversion were hydrolysis temperature of 50.8℃,pH value of 5.16,hydrolysis time of 2.5 h.Under those conditions,the total aglycones quality obtained from 15 mg sea buckthorn leaf extract was 0.180 1 mg and the conversion rate to flavonoids aglycones was 62%.

关 键 词：聚半乳糖醛酸酶 沙棘叶 黄酮 响应面 转化率 

分 类 号：TS201.1[轻工技术与工程—食品科学] Q556.2[轻工技术与工程—食品科学与工程]