低频超声辐射对融合蛋白PTD-SOD渗透离体皮肤的影响  

Effect of low-frequency ultrasound on trans-dermal permeation of fusion protein PTD-SOD in vitro

作　　者：赵林[1,2] 谢艳招[1] 潘剑茹[2] 刘树滔[2] 

机构地区：[1]福建师范大学闽南科技学院,泉州362332 [2]福州大学生物工程研究所,福州350002

出　　处：《辐射研究与辐射工艺学报》2011年第2期99-103,共5页Journal of Radiation Research and Radiation Processing

基　　金：福建省教育厅B类项目(JB09079)资助

摘　　要：通过小鼠的离体皮肤渗透,研究了低频超声(40 kHz)辐射对融合了蛋白转导域的超氧化物歧化酶(Protein transduction domain-superoxide dismutase,PTD-SOD)体外经皮渗透的影响。结果表明,透皮8 h时,低频超声与氮酮(Azone)的增渗比分别为3.40和2.52;低频超声作用下,药物初始pH=6.0时,接收池中的SOD活性最高,可达1598.00 U;药物浓度效应符合Fick扩散定律,且药物渗透系数为0.011。这些结果说明,低频超声能显著提高PTD-SOD的经皮渗透速率,且效果优于Azone;药物溶液的初始pH值及药物初始浓度均能影响PTD-SOD的透皮速率,其最佳pH值及最佳给药浓度分别为6.0和15.00 kU·mL-1。By means of trans-dermal permeation on rat skin in vitro,the effect of low-frequency ultrasound（40 kHz） on trans-dermal permeation of（Protein transduction domain-superoxide dismutase,PTD-SOD） has been inves-tigated.The results show that the enhancement ratios of ultrasound and Azone are 3.40 and 2.52,respectively,when the trans-dermal permeation time is 8 h.In the presence of low-frequency ultrasound,when the initial pH value of drug solution is 6.0,the activity of PTD-SOD in receiver cell reaches 1598.00 U,the maximum value.Under the ac-tion of low-frequency ultrasound,the trans-dermal permeation of drug varies as a function of the initial PTD-SOD concentration according with a Fick-type scattering law,and the coefficient of permeation is 0.011.These results demonstrate that Low-frequency ultrasound can significantly improve trans-dermal transport of PTD-SOD,and the effect is superior to Azone.Both of the initial pH value of drug solution and the initial PTD-SOD concentration can affect the permeation rate of the PTD-SOD.And the best initial pH value and drug concentration is 6.0 and 15.00kU·mL-1,respectively.

关 键 词：经皮渗透 低频超声 蛋白转导域 超氧化物歧化酶 

分 类 号：R94[医药卫生—药剂学] R979.19[医药卫生—药学]