两步超滤法分离鸡蛋清中卵转铁蛋白的研究  

Separation of ovotransferrin from chicken egg white using two-stage ultrafiltration technique

作　　者：袁小军[1] 马美湖[1] 黄群[1] 吴潇[1] 

机构地区：[1]华中农业大学国家蛋品加工技术研发分中心,湖北武汉430070

出　　处：《食品与机械》2011年第2期125-128,137,共5页Food and Machinery

基　　金：现代农业产业技术体系建设专项资金(编号:nycytx-41-g22)

摘　　要：以配有聚砜树脂衍生物膜的超滤装置为载体,结合透析、冷冻干燥处理,发展一种从蛋清中分离提取卵转铁蛋白经济简单的方法,并研究操作压力、搅拌速度、溶液pH值、稀释倍数等操作参数对膜通量的影响,并优化工艺。结果表明,两步超滤法分离鸡蛋清中卵转铁蛋白的最佳条件是操作压力0.15 MPa,蛋清液和溶剂按体积比1∶10进行稀释,搅拌速度125 r/min,体系pH值为7。该条件下得到卵转铁蛋白纯度为29.6%。Ultrafiltration device fitted with polyether sulfone membrane were used as crosslinking agent,developing a simple and economical protocol for separating ovotransferrin from egg white.The effects have been studied of trans-membrane pressure,stirrer speed,solution pH and dilution of raw materials on permeate flux.Results of the investigation showed that the best conditition of separating ovotransferrin from egg white using two-stage ultrafiltration technique were： trans membrane pressure 0.15 MPa,chicken egg white-solvent ratio 1∶10,stirring speed 125 r/min,pH of the solution is 7.Under those conditions,the purity of obtained ovotransferrin is 29.6%.

关 键 词：鸡蛋清蛋白 卵转铁蛋白 超滤 聚砜树脂衍生物膜 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]