HPLC法测定七制香附丸中α-香附酮的含量  

Content Determination of α-Cyperone in Qizhi Xiangfu Pills by HPLC

作　　者：吕霞[1] 苟琼心[1] 李陈波[1] 田友清[1,2] 丁平[1] 

机构地区：[1]江苏联合职业技术学院连云港中医药分院,连云港市222006 [2]中国药科大学,南京市210009

出　　处：《中国药房》2011年第16期1507-1508,共2页China Pharmacy

基　　金：连云港市卫生局医药卫生科研课题(08053)

摘　　要：目的:建立测定七制香附丸中α-香附酮含量的方法。方法:采用高效液相色谱法。色谱柱为InertsilODS-SP柱,流动相为甲醇-水(77:23),流速为1.0mL·min-1,检测波长为254nm,柱温为30℃,进样量为10μL。结果:α-香附酮进样量在0.03784～0.7568μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为98.07%,RSD=1.19%。结论:该方法简便、准确、可控,可作为七制香附丸的质量控制方法。OBJECTIVE：To develop the method for the content determination of α-Cyperone in Qizhi xiangfu pills.METHODS：HPLC method was adopted.The determination was performed on Inertsil ODS-SP column with mobile phase consisted of methanol-water（77：23）at a flow rate of 1.0 mL·min-1.The determined wavelength was set at 254 nm and the column temperature was 30 ℃.The injection volume was 10 μL.RESULTS：The linear ranges of α-cyperone was 0.037 84～0.756 8 μg with an average recovery of 98.07%（RSD=1.19%）.CONCLUSION：This metohd is convenient,rapid and accurate.It can be used for quality control of Qizhi xiangfu pills.

关 键 词：高效液相色谱法 Α-香附酮 七制香附丸 含量测定 

分 类 号：R917[医药卫生—药物分析学]