细菌素Bacteriocin E50-52(H)基因设计及其毕赤酵母表达载体构建  

Gene Design of Bacteriocin E50-52 and Construction of its Pichia pastoris Expression Vector

作　　者：余占桥[1] 马青山[1] 韩冰[1] 张日俊[1] 

机构地区：[1]中国农业大学饲料生物技术实验室,动物营养学国家重点实验室,北京100193

出　　处：《中国畜牧兽医》2011年第4期77-79,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30972124)

摘　　要：设计及合成细菌素Bacteriocin E50-52(H)基因,克隆到组成型分泌表达质粒pGAPZαA中构建重组质粒pGAPZα-Bacteriocin E(H),经PCR、测序验证正确后,电转化整合到毕赤酵母基因组,基因组PCR、测序验证结果表明成功构建了细菌素组成型重组表达载体,为在毕赤酵母中表达奠定了基础。Bacteriocin E50-52 gene was designed according to its amino acid sequence and cloning site of constitutive secreted plasmid pGAPZαA,then synthesized,inserted into pMD18-T.Recombinant pGAPZα-Bacteriocin E was constructed by ligating the digested target gene and pGAPZαA with XhoⅠ and XbaⅠ.Recombinant Pichia pastoris was constructed by transforming linearized recombinant plasmid in strain SMD1168.Sequencing result of recombinant vector and genome of Pichia pastoris indicated this expression plasmid was successfully constructed.

关 键 词：BacteriocinE50-52 基因设计 毕赤酵母 组成型表达载体 

分 类 号：Q78[生物学—分子生物学]