冷应激后民猪肝素结合性表皮生长因子基因表达研究  

Study on Expression of Min Pig Hepafin-binding Epidermal Growth Factor-like Gene in Cold Stress

作　　者：张冬杰[1] 刘娣 汪亮[1] 别墅[1] 孙洪涛[1] 杨国伟[1] 

机构地区：[1]黑龙江省农业科学院,黑龙江哈尔滨150086

出　　处：《中国畜牧兽医》2011年第4期98-100,共3页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划重点项目(2008BADB2B01)

摘　　要：肝素结合性表皮生长因子(hepafin-binding epidermal growth factor-like,HB-EGF)是表皮生长因子(epidermal growth factor,EGF)家族的成员之一。本研究采用Real-time PCR分析了在民猪冷诱导后该基因在骨骼肌内的表达变化情况。结果表明,低温组(-20℃)的HB-EGF基因的表达水平与常温组(10℃)相比出现了极显著下降(P<0.01)。据此推测,冷应激抑制了HB-EGF基因的表达,也间接抑制了民猪的正常生长。Hepafin-binding epidermal growth factor-like（HB-EGF） is one of the epidermal growth facto family.In this study,Real-time PCR method was used to detect and analysis the expression of HB-EGF gene in Min pig skeletal muscle after cold-induced.The results showed that the low-temperature group（-20 ℃） of HB-EGF gene expression levels compared with the normothermia group（10 ℃） appeared very significantly decreased（P0.01）.As above,cold stress inhibits the expression of HB-EGF gene,but also indirectly inhibit the normal growth of Min pig.

关 键 词：民猪 冷应激 HB-EGF REAL-TIMEPCR 

分 类 号：Q786[生物学—分子生物学]