免疫色谱法及酶联免疫法检测甲壳类食物过敏原  

Determination of Food Allergen-Crustaceans by Immuno Chromato and Enzyme Immuno Assay

作　　者：王婧[1] 王锡昌[1] 相里辰[1] 奚印慈[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《现代食品科技》2011年第4期482-485,共4页Modern Food Science and Technology

摘　　要：比较了世界各国食物过敏原标示制度及主要过敏原物质的检测方法。使用免疫色谱法(简易试纸卡盒定性法)和酶联免疫法(ELISA试剂盒定量法)对中国国内生产销售的预包装食品中食物过敏原物质-甲壳类原肌球蛋白的含量进行了定性、定量检测。结果表明两种方法有很好的相关性。免疫色谱法(简易试纸卡盒定性法)操作简单、快速便利,可用于食品工厂、餐饮业等的快速检测。酶联免疫法(ELISA试剂盒定量法)快速、定量准确、灵敏度高,可用于科学研究及高灵敏度、高精确度的检测。本实验选用样品中的过敏原物质-甲壳类的测定结果与包装上配料表中标示内容相符。Food allergen labeling legislation overseas and main crustacean determination methods were compared.Crustaceans in packaged processed foods that made and sold in Shanghai were analyzed by Immuno chromato and enzyme Immuno Assay Kit.The results showed two methods have good correlation.Immuno chromato is suitable for fast testing in Factory or fast food industry.Enzyme Immuno Assay is suitable for research and quantitative Analysis.The results of crustacean tropomyosin in 12 various foods showed these package labelings are correct.

关 键 词：食物过敏原物质 甲壳类 免疫色谱法 酶联免疫法 

分 类 号：TS207.4[轻工技术与工程—食品科学]