CD3^+CD56^+NKT细胞及亚群在甲状腺癌患者的表达及临床意义  

Expression and clinical significance of CD3^+CD56^+NKT cells and its two subtypes in the peripheral blood of the patients with papillary thyroid carcinoma

作　　者：谢澎[1] 苏文[2] 王艳峰[2] 贾莉[2] 丁弋[2] 武帅[3] 武乃旺[3] 

机构地区：[1]山西医科大学第二临床医学院,太原030013 [2]山西省肿瘤医院肿瘤研究所免疫室 [3]山西省肿瘤医院头颈科

出　　处：《中国医疗前沿》2011年第5期13-14,共2页China Healthcare Innovation

摘　　要：目的本研究通过测定甲状腺乳头状癌患者外周血中CD3+CD56+NKT细胞及其亚型CD3+CD56+CD4+NKT、CD3+CD56+CD8+NKT细胞的表达,初步探讨其在甲状腺肿瘤中的免疫调控作用。方法采用流式细胞术分析53例甲状腺乳头状癌患者及37例健康对照组外周静脉血中NKT细胞以及两种亚型的表达。结果甲状腺乳头状癌患者外周静脉血中NKT细胞表达率明显高于对照组(P<0.01);CD4+NKT细胞表达率也高于对照组(P<0.01),而CD8+NKT细胞表达率与健康对照组之间无统计学差异(P>0.05);甲状腺乳头状癌TNM分期中,三种细胞的表达率在I、Ⅱ、Ⅲ期之间无统计学差异(P>0.05);结论 NKT细胞在甲状腺乳头状癌患者中的高表达提示其在甲状腺乳头状癌免疫调控中的重要作用,而发挥这一作用的主要为CD4+NKT细胞;NKT细胞及其两种亚型的测定对甲状腺乳头状癌临床分期的辅助诊断意义不大。Objective The aim of this research is to preliminarily explore the immunoregulation effect of NKT cells and its two subtypes： CD3＋CD56＋CD4＋NKT and CD3＋CD56＋CD8＋NKT cells on thyroid neoplasm through determination of their expression in peripheral blood of patients with thyroid papillary carcinoma.Methods NKT cells and its two subtypes in peripheral venous blood were analyzed in 53 patients with papillary thyroid carcinoma and 37 healthy subjects by flow cytometry.Results Expression rate of NKT cells in peripheral venous blood was higher significantly in patients with thyroid papillary carcinoma than normal control group（P〈0.01） and CD4＋NKT also showed similar trend（P〈0.01） while there was no significant difference in CD8＋NKT cells between healthy control group and patients with papillary thyroid carcinoma（P〈0.05）.The expression of peripheral NKT cells and CD4＋ as well as CD8＋NKT cells have no statistical difference among patients with stage Ⅰ,Ⅱ and Ⅲ thyroid papillary carcinoma（P〈0.05）.Conclusions The high expression of peripheral NKT cells in patients with papillary thyroid carcinoma suggested that it may play roles in immunoregulation of papillary thyroid carcinoma and CD4＋NKT cells play an important role.The determination of NKT cells and its two subtypes have no significant effect on the clinical stage of papillary thyroid carcinoma.

关 键 词：NKT细胞 CD4+NKT细胞 CD8^+NKT细胞 甲状腺肿瘤 免疫 

分 类 号：R736.1[医药卫生—肿瘤]