燕窝中唾液酸含量的分光光度测定方法  

Determination of sialic acid in bird nest by spectrophotometer

作　　者：李敏[1] 黄华军[1] 奚星林[1] 梁瑞婷[1] 李双[1] 李杏[2] 

机构地区：[1]广东检验检疫技术中心,广州510623 [2]广东药学院,广州510623

出　　处：《中国卫生检验杂志》2011年第3期598-600,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:研发燕窝中唾液酸含量的测定方法。方法:先用乙酸水解法把燕窝中的唾液酸释放出来,唾液酸与酸性茚三酮形成稳定的黄色物质,后用分光光度法进行定量分析。结果:本文主要研究了酸水解方法中酸的种类、酸的浓度、时间等因素对结果测定的影响。结论:燕窝先经前处理,再加酸水解(时间10 min,水解试剂为50%醋酸),后在470 nm处测量吸光度来进行定量。Objective：Devlepment of determination of sialic acid in bird nest.Methods：Acetic acid was used to hydrolyze bird nest and get sialic acid.Sialic acid can react with ninhydrin to form yellow substance,which can be measured by its absorption spectrophotonletry.Results：Kinds of acid、concentration of acid and hydrolyzation exausting time,were planed to research their effect on the results.Conclusion：The better determination method could be formed.Bird nest should be prepared firstly,than be hydrolyzed by 50% Acetic acid solution in 10 min,finally be measured by its absorbing wavelength on the 470 nm.

关 键 词：燕窝 唾液酸 茚三酮 分光光度法 

分 类 号：O657.32[理学—分析化学]