Decorin mRNA、p57^(KIP2) mRNA和TGF-β在肺癌中的表达及临床意义  

Study on expressions of Decorin mRNA、 p57^(KIP2) mRNA and TGF-β1 and their clinical significance in lung cancer

作　　者：戎彪学[1] 蔡曦光[2] 刘华[2] 杨拴盈[1] 

机构地区：[1]西安交通大学第二附属医院呼吸科,陕西省西安市224006 [2]兰州大学临床医学院呼吸科,甘肃省兰州市730000

出　　处：《实用老年医学》2011年第2期137-139,共3页Practical Geriatrics

摘　　要：目的研究Decorin mRNA、p57KIP2mRNA和转化生长因子-β1(TGF-β1)在肺癌中的表达情况与临床病理特征的关系。方法用原位杂交法检测64例肺癌患者及12例癌旁正常肺组织Decorin mRNA和p57KIP2mRNA的表达,免疫组化法(S-P法)检测64例肺癌患者及12例癌旁正常组织TGF-β1的表达。结果 DecorinmRNA在正常肺组织的表达率为66.7%(8/12),明显高于肺癌组织15.6%(10/64)(P<0.05)。鳞癌中p57KIP2mRNA的阳性表达率是68.4%(13/19),表达分别高于腺癌、大细胞癌、小细胞癌和肺泡细胞癌(P<0.05)。TGF-β1在小细胞癌中的阳性表达率是33.3%(3/9),与鳞癌、腺癌、大细胞癌及肺泡细胞癌相比呈低表达(P<0.05)。无淋巴结转移的肺癌标本中Decorin mRNA和p57KIP2mRNA的表达呈增高趋势(P<0.05)。Decorin mRNA、p57KIP2mRNA和TGF-β1的表达之间没有相关性(P>0.05)。结论 Decorin mRNA、p57KIP2mRNA和TGF-β1的表达可能与肺癌的组织学类型和肺癌的转移有关。Objective To investigate the expressions of Decorin mRNA、p57KIP2 mRNA and TGF-β1 and to analyze their relationships with pathogenesis and clinical characteristics in lung cancer.Methods The expressions of Decorin mRNA and p57KIP2 mRNA of 64 cases of lung cancer and 12 cases of normal lung tissues adjacent to tumor tissues were examined by in situ hybridization.and expression of TGF-β1 of 64 cases of lung cancer and 12 cases of normal lung tissues were examined by immunohistochemistry（streptavidin-perosidase）.Results The normal tissues showed a higher expre-ssion of Decorin mRNA（66.7%,8/12） than the tumor tissues（15.6%,10/64）（P0.05）.The expression rate of p57KIP2 mRNA in squamous cell carcinoma of the lung（LSCC） was 68.4%（13/19）,being higher than adenocarcinoma of the lung（LAC）,large cell lung adenocarcinoma（LCLC）,small cell lung adenocarcinoma（SCLS）and alveolar carcinoma（P0.05）.The expression rate of TGF-β1 in SCLS was lower than LAC,LCLC,LSCC and alveolar carcinoma.In lung cancer without lymph node metastasis,the expression of Decorin mRNA and p57KIP2 mRNA increased significantly（P0.05）.Conclusions These results suggest that Decorin gene,p57KIP2 gene and TGF-β1 are closely related to the lung cancer metastasis and histological classification.

关 键 词：肺癌 DECORIN MRNA P57KIP2 MRNA 转化生长因子-β1 组织芯片 

分 类 号：R734.2[医药卫生—肿瘤]