HPLC法测定不同品种及段木栽培灵芝子实体中麦角甾醇含量  

Determination of Ergosterol in Ganoderma lucidum from Different Varieties and Cultured Tree Species by HPLC

作　　者：刘京晶[1] 黄文华[1] 吕明亮 斯金平[3] 郭宝林[1] 李世洁[1] 

机构地区：[1]中国医学科学院/北京协和医学院药用植物研究所,北京100193 [2]浙江省丽水市林业科学研究所,浙江丽水323000 [3]浙江林学院林业与生物技术学院,浙江临安311300

出　　处：《中药材》2011年第2期187-190,共4页Journal of Chinese Medicinal Materials

基　　金：浙江省科技项目(2004C33056)

摘　　要：目的:以麦角甾醇为测定指标,建立灵芝子实体质量评价的HPLC方法,对不同品种及段木栽培灵芝子实体中麦角甾醇的含量进行比较研究。方法:采用HPLC法定量分析17个不同品种及14个不同段木树种的栽培灵芝子实体。色谱柱:Diamonsil C18,流动相:乙腈,流速:1.0 mL/min,柱温:40℃,检测波长:282 nm。结果:确定了灵芝子实体中麦角甾醇的提取方法和HPLC含量测定方法。不同品种麦角甾醇含量范围为0.093%～0.243%,其中韩芝-2号、圆芝、明-1、野生1号同时具有较高的麦角甾醇含量和转化率;不同段木树种麦角甾醇含量范围为0.080%～0.227%,以白栎、杨梅的麦角甾醇含量及转化率均较高。结论:本方法操作简便、结果准确,可用于灵芝子实体中麦角甾醇的含量测定和灵芝质量评价研究,为人工栽培灵芝的品种及段木树种的选择提供了参考。Objective：HPLC method for determining ergosterol was established to evaluate the quality of Ganoderma lucidum and to analyze of the ergosterol in different varieties and cultrued tree species.Methods：Samples from 17 different varieties and 14 different tree species were quantified by HPLC.The RP-HPLC was conducted on Diamonsil C18 column with acetonitrile as the mobile phase at 40 ℃.The flow rate was 1.0 mL/min;and the detection wavelength was 282 nm.Results：The fluctuations of ergosterol content was 0.093%~0.243% among different varieties and 0.080%~0.227% among different tree species.Hanzhi-2,Yuanzhi,Ming-1,Yesheng-1 hao were excellent at both ergosterol content and conversion rate among all the varieties,and Bai Li,Yang Mei have comparatively more ergosterol and high conversion rate among all the tree species in this study.Conclusions：This method is simple,sensitive and accurate.It could be used to determine the contents of ergosterol and the evaluation of the quality in Ganoderma.It also provides references for choosing varieties and tree species when culture Ganoderma.

关 键 词：灵芝子实体 麦角甾醇 HPLC 品种 段木栽培 

分 类 号：R282.4[医药卫生—中药学]