直杆桉果实提取物体外抗单纯疱疹病毒和乙型肝炎病毒的实验研究  

In vitro Study on the Anti-HSV-1 and HBV Activities of Extracts from the Fruit of Eucalyptus maidenii

作　　者：鞠怀强[1,2,3] 王沙燕[1,2] 裴赢 向阳飞[3] 李深[3] 张颖君[4] 杨崇仁[4] 王一飞[3] 

机构地区：[1]暨南大学第二临床医学院 [2]深圳市人民医院,广东深圳518020 [3]暨南大学生物医药研究开发基地,广东广州510632 [4]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《中药材》2011年第2期242-245,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金委员会-广东省人民政府自然科学联合基金(U0632010);植物化学与西部植物资源持续利用国家重点实验室自主课题(O807E21211);开放课题(O807B11211)

摘　　要：目的:研究3种不同溶剂的直杆桉果实提取物体外抗单纯疱疹病毒Ⅰ型(HSV-1)和乙型肝炎病毒(HBV)的活性。方法:采用MTT法检测药物毒性,CPE法与空斑减数实验检测药物抗HSV-1活性;采用ELISA法检测药物对HepG2.2.15细胞分泌的乙型肝炎表面抗原(HBsAg)和乙型肝炎E抗原(HBeAg)的抑制作用。结果:直杆桉叶果实的醋酸乙酯提取物(P18-E1)和甲醇提取物(P18-E2)无明显抗HSV-1的活性,水提物(P18-E3)能明显抑制HSV-1的致病变作用,其TC50为69.20 mg/mLI,C50为126.77μg/mL,治疗指数为545.87;3种不同溶剂的提取物对HepG2.2.15细胞分泌的HBsAg和HBeAg均无明显抑制作用。结论:直杆桉果实的水提物(P18-E3)具有显著的抗HSV-1活性,主要是对病毒有直接灭活作用,也能影响病毒对细胞的吸附。初步推测其抗HSV-1活性的机理是影响病毒的囊膜结构从而使之失活。Objective：To investigate the antiviral activities of three kinds of extracts from the fruit of Eucalyptus maidenii against herpes simplex virus type1 and Hepatitis B Virus.Methods：Cytotoxicity of extracts on Vero cell lines were estimated using MTT method and anti-HSV-1 activity was observed and determined with CPE and plaque reduction assay.The inhibitory effects of extracts on HBsAg and HBeAg secretion in HepG2.2.15 cell culture were detected using ELISA.Results：Aqueous extract（p18-E3）had conspicuous anti-HSV-1 activity,the IC50 was 126.77 μg/mL,but the EtOAc extracts（p18-E1）and MeOH extracts（p18-E2）showed little anti-HSV-1 activity.None of these extracts had significant inhibitory eflect on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.Conclusion：Aqueous extract（p18-E3）from the fruit of Eucalyptus maidenii has conspicuous anti-HSV-1 activity.It could inactivate virus directly,and inhibit virus attachment,but had no influence on virus penetration.The mechanism that p18-E3 inactivates virus might involve in viral envelope alteration.

关 键 词：直杆桉果实 单纯疱疹病毒Ⅰ型(HSV-1) 乙型肝炎病毒(HBV) HEPG2.2.15细胞 

分 类 号：R285.5[医药卫生—中药学]