余甘子叶化学成分没食子酸诱导人肝癌细胞株BEL-7404凋亡机制的研究  

Study of Galic Acid Extracted from the Leaves of Phyllanthus emblica on Apoptotic Mechanism of Human Hepatocellular Carcinoma Cells BEL-7404

作　　者：黄金兰[1] 钟振国[1] 

机构地区：[1]广西中医学院新药研究开发中心,广西南宁530001

出　　处：《中药材》2011年第2期246-249,共4页Journal of Chinese Medicinal Materials

基　　金：广西科技学技术厅项目(桂科基0639051;桂科攻0718002-2-1);广西高校人才小高地建设创新团队资助计划;广西研究生教育创新计划资助项目(桂中研「2008」24号);广西科技厅自然基金项目(桂科自0728175)

摘　　要：目的:探讨余甘子叶化学成分没食子酸诱导人肝癌细胞株BEL-7404产生凋亡的机制。方法:采用PI和Hoechst33342双染色法流式检测对人肝癌细胞株BEL-7404各周期的影响;细胞荧光免疫染色法和Western blot法检测Bax和Bcl-2蛋白表达的变化。结果:PI和Hoechst33342双染色法分析结果表明,余甘子叶化学成分没食子酸作用BEL-7404细胞72 h后,G2/M期细胞数增多,呈现细胞阻滞现象,并伴有大量的细胞凋亡。荧光免疫染色显示,药物作用人肝癌细胞株BEL-7404 72 h后Bax蛋白表达升高,Bcl-2蛋白表达则降低。Western blot法实验亦显示Bax蛋白表达升高。结论:余甘子叶化学成分没食子酸能阻滞G2/M期细胞,并通过上调Bax和下调Bcl-2蛋白表达,导致线粒体膜电位的下降,进而激活Caspase,最终导致细胞死亡,可能是其诱导人肝癌细胞株BEL-7404凋亡的分子机制之一。Objective：To explore the mechanism of apoptosis of human hepatocellular carcinoma cells BEL-7404 induced by Galic acid extracted from leaves of Phyllanthus emblica.Methods：PI/Hoechst33342 double staining method was utilized to observe the influence on cell life cycle.The expressions of Bax and Bcl-2 protein were determined by fluorescence immunostaining and Western blot.Results：The results from PI/Hoechst33342 double staining method indicated that the percentage of cells G2/M phases increased after treated for 72 h,the phenomenon of blockage appeared and cell death was induced.Fluorescence immunostaining showed that the expression of Bax was up-regulated,and the expression of Bcl-2 was down-regulated.Western blot also showed that the the expression of Bax was up-regulated.Conclusion：The mechanism of human hepatoma BEL-7404 cells apoptosis induced by Galic acid from leaves of Phyllanthus emblica may be blocking G2/M period in cell life cycle,up-regulating the expression of Bax and down-regulating the expression of Bcl-2,that can decrease membrane potential of mitochondria,and triggered the caspases of activation of cascade and induced cell death.

关 键 词：余甘子叶 没食子酸 BEL-7404细胞 细胞凋亡机制 

分 类 号：R285.5[医药卫生—中药学]