Effect of acupuncture on antioxidant capacities and protection of vascular endothelium in hyperlipidemia mice  

针刺对高脂血症小鼠抗氧化能力及血管内皮保护功能的影响(英文)

作　　者：王志波[1] 肖红玲[2] 杨继军[2] 王丽芳[1] 张瑞娟[3] 肖丽梅 杨云峰[2] 

机构地区：[1]华北科技学院医院 [2]河北医科大学中医学院针灸系,石家庄050091 [3]河北工程大学医学院 [4]河北新乐市中医院

出　　处：《World Journal of Acupuncture-Moxibustion》2011年第1期38-43,共6页世界针灸杂志（英文版）

基　　金：Supported by Key Project Foundation of Hebei Administration of TCM:2009058

摘　　要：Objective To observe the effect and explore the mechanism of acupuncture on hyperlipemia in mice. Methods One hundred and twenty male Kunming mice of clean grade were randomly divided into a normal group （40 mice）, a model group （40 mice）, an acupuncture group （20 mice）, and a drug group （20 mice）. The hyperlipidemia model was prepared in the latter 3 groups by feeding with high fat diet. After the successful modeling, bilateral ＂Fenglong＂ （丰隆 ST 40）, ＂Quchi＂ （曲池 LI 11）, and ＂Sanyinjiao＂ （三阴交 SP 6） were selected in the acupuncture group and Han＇s Acupuncture Stimulator was connected to intervent for 10 rain, once daily, for 10 successive days. Mice in the drug group were orally given Simvastatin, once daily, for 10 successive days. No intervention was given to the normal group and the model group. Serum contents of total cholesterol （TC）, triglyeeride （TG）, low-density lipoprotein cholesterol （LDL-C）, high-density lipoprotein cholesterol （HDL-C）, malondialdehyde （MDA）, nitric oxide （NO）, endothelin （ET）, and the activity of superoxide dismutase （SOD） were detected. Results The serum contents of TG, TC and LDL-C in the model group detected on the 15th day were all higher than those of the normal group [（1.31±0.32） mmol/L vs （0.70±0.21） retool/L, （5.72±0.85） mmol/L vs （3.08±0.74） retool/L, （2.68±0.55） mmol/L vs （1.83±0.36） retool/L, respectively]. The serum content of HDL-C in the model group was lower than that in the normal group [（0.60±0.26） mmol/L vs （0.94±0.30） mmol/L], showing successful modeling. On the lOth day of successful modeling, the serum contents of MDA and ET in the acupuncture group were lower than those in the model group [（21.58±3.54） p, mol/mL vs （29.94±2.79） p, mol/mL, （44.51±5.23） pg/mL vs （67.07±11.98） pg/mL]. The serum SOD activities and NO content in the acupuncture group were higher than those in the model gropu [（264.57±14.46） U/mL vs （目的:观察针刺对高脂血症小鼠的作用及作用机制。方法:将昆明雄性清洁级小鼠120只,随机分为正常组(40只)、模型组(40只)、针刺组(20只)和药物组(20只)。后3组采用高脂饮食饲料喂养制备高脂血症小鼠模型。造模成功后,针刺组针刺双侧"丰隆""曲池""三阴交"穴后接通韩氏电针仪干预10 min,每日1次,连续干预10 d;药物组灌服辛伐他汀药物,每日1次,连续10 d;其他两组不予干预。检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙二醛(MDA)、一氧化氮(NO)、内皮素(ET)含量和超氧化物歧化酶(SOD)活力。结果:第15天检测模型组小鼠血清TG 为(1.31±0.32)mmol/L、TC为(5.72±0.85)mmol/L、LDL-C为(2.68±0.55)mmol/L,含量均高于正常组的TG(0.70±0.21)mmol/L、TC(3.08±0.74)mmol/L、LDL-C(1.83±0.36)mmol/L含量;模型组小鼠血清HDL-C含量是(0.60±0.26)mmol/L,低于正常组HDL-C含量(0.94±0.30)mmol/L,表明造模成功。造模成功后针刺组针刺10 d,小鼠血清MDA为(21.58±3.54)μmol/mL、ET 为(44.51±5.23)pg/mL,其含量均低于模型组小鼠血清的MDA(29.94±2.79)μmol/mL、ET(67.07±11.98)pg/mL含量;针刺组小鼠血清SOD活力为(264.57±14.46)U/mL、NO 含量为(85.02±10.06)μmol/L,均高于模型组的SOD活力(222.66± 25.48)U/mL、NO含量(63.83±9.19)μmol/L。结论:针刺可降低高脂血症小鼠血清MDA、ET含量,提高SOD活力和NO含量,表明该针刺配穴法能够抵抗高脂血症小鼠脂质过氧化,清除机体自由基,调节和改善自由基代谢平衡,并具有保护血管内皮功能的作用。

关 键 词：Acupuncture Therapy HYPERLIPIDEMIA ANTIOXIDATION Free Radical Vascular Endothelial Cell 

分 类 号：R245[医药卫生—针灸推拿学]