人参皂甙Rd抑制大鼠局灶性脑缺血后趋化因子CXCL1和γ-干扰素的蛋白表达  

Ginsenoside-Rd Inhibits CXCL1 and Interferon-γ Protein Expressions in Rats after Focal Cerebral Ischemia

作　　者：张云霞[1] 赵钢[1] 史明[1] 周林甫[1] 

机构地区：[1]第四军医大学附属西京医院神经内科,陕西西安710032

出　　处：《现代生物医学进展》2011年第6期1059-1062,共4页Progress in Modern Biomedicine

摘　　要：目的:研究人参皂甙Rd(Ginsenoside-Rd,GS-Rd)在大鼠局灶性脑缺血后对炎症趋化因子CXCL1和γ-干扰素(Interferon-γ,IFN-γ)的影响。方法:将SD大鼠随机分为5组:正常组(n=5),假手术组(n=5),GS-Rd对照组(n=5),大脑中动脉栓塞模型(MCAO)组(n=20),MCAO+GS-Rd组(n=20)。正常组不做任何处理;假手术组进行大脑中动脉栓塞手术,但不插入栓线;GS-Rd对照组给予腹腔注射10 mg/Kg GS-Rd,不进行手术;MCAO组(n=20)和MCAO+GS-Rd组(n=20)进行大脑中动脉栓塞手术,术后2小时拔出栓线,MCAO+GS-Rd组在术前15分钟腹腔注射10 mg/Kg GS-Rd。在12小时、1天、3天、7天四个时间点分别提取脑组织蛋白,通过液相芯片技术检测CXCL1,IFN-γ含量。结果:正常组,假手术组和GS-Rd对照组组间CXCL1,IFN-γ含量无统计学差异;与三个对照组相比,MCAO组和MCAO+GS-Rd组中CXCL1,IFN-γ蛋白含量均有明显增加(P<0.05);而与MCAO组相比,MCAO+GS-Rd组CXCL1,IFN-γ的生成明显减少(P<0.05)。结论:10 mg/Kg GS-Rd预处理可有效抑制大鼠短暂性脑缺血后CXCL1,IFN-γ的生成;通过抑制炎症反应,GS-Rd可能在神经保护中发挥重要的作用。Objective：To investigate the effect of Ginsenoside-Rd（GS-Rd） on protein expression of CXCL1 and IFN-γ（interfer-on-γ,IFN-γ）in rats after focal cerebral ischemia.Methods：A total of 55 rats were randomly divided into the five groups：blank group（n=5）;Sham group（n=5）,with surgery but no occlusion;GS-Rd group（n=5）,with 10 mg/kg GS-Rd treatment but no surgery;middle cere-bral artery occlusion（MCAO） group（n=20）,with vehicle application before surgery;MCAO＋GS-Rd group（n=20）,with 10 mg/kg GS-Rd treatment before MCAO.After 2 h of occlusion,the suture was carefully removed to restore blood flow.Then the brain protein were ex-tracted respectively at 12hour,1day,3day,7day and were tested by LUMINEX 200.Results：Our results showed that the protein levels of CXCL1 and IFN-γ were significantly increased after MCAO insult at all time points（p0.05）.Pretreatment of 10 ml/kg GS-Rd inhibited MCAO-induced protein expressions of CXCL1 and IFN-γ（p0.05）.Conclusion：These results suggest that neuroprotection of GS-Rd following cerebral ischemia may be at least due to inhibition of the proteins expression of CXCL1 and IFN-γ.

关 键 词：人参皂甙RD 液相芯片 趋化因子CXCL1 Γ-干扰素 

分 类 号：Q95-3[生物学—动物学] R743.31[医药卫生—神经病学与精神病学]