大鼠口轮匝肌和腓肠肌乙酰胆碱受体密度及其与罗库溴铵亲和力的比较  

The density of acetyicholine receptor in orbicular muscle of mouth and gastrocnemius muscle and the difference in their affinity with rocuronium

作　　者：周汝元[1] 徐静[1] 陈莲华[1] 金慰芳[2] 钟高仁[2] 徐培康[2] 

机构地区：[1]复旦大学眼耳鼻喉科医院麻醉科,上海市200031 [2]复旦大学放射医学研究所

出　　处：《中华麻醉学杂志》2011年第1期34-36,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金面上项目（30872428）

摘　　要：目的 比较大鼠口轮匝肌和腓肠肌乙酰胆碱受体密度及其与罗库溴铵的亲和力,探讨口轮匝肌和腓肠肌对罗库溴铵敏感性差异的机制.方法 清洁级成年雄性SD大鼠8只,体重180～220 g,取口轮匝肌和腓肠肌各一大束,每束分离出等粗的6束,其中1束用于测终板表面积,另外5束分别用于测定不同浓度罗库溴铵[0（未加入罗库溴铵）、2.5、5.0、7.5、10.0μg/ml]时乙酰胆碱受体数量,计算乙酰胆碱受体密度和乙酰胆碱受体与罗库溴铵的亲和力.结果 与口轮匝肌比较,腓肠肌乙酰胆碱受体密度降低,乙酰胆碱受体与罗库溴铵亲和力升高（P＜0.05）.结论 与口轮匝肌比较,大鼠腓肠肌乙酰胆碱受体密度降低,乙酰胆碱受体与罗库溴铵亲和力升高,这种差异可能是造成口轮匝肌和腓肠肌对罗库溴铵敏感性差异的机制.Objective To compare the density of acetylcholine receptor （AchR） in orbicular muscle of mouth and gastrocnemius muscle and their affmity with rocuronium, trying to elucidate the mechanism for the difference in the sensitivity of the muscles innervated by facial and peripheral nerve respectively to muscle relaxant.Methods Eight pathogen-free adult male SD rats weighing 180-220 g were used in this study. Muscle strips were isolated from orbicular muscle of mouth and gastrocnemius muscle. Each muscle strip was further divided into 6 smaller and slender strips of same size using dissection microscope. One strip was stained with acetylcholinesterase to measure end-plate surface area （ESA）. The other 5 strips were exposed to different concentrations of rocuronium （0, 2.5, 5.0, 7.5, 10.0μg/ml）. The mean density of AchR at end-plate was obtained by AchR0/ESA. （AchR0 was defined as the number of AchR per end-plate without being exposed to rocuronium. AchRE was defined as the number of free AchR per end-plate after being exposed to different concentrations of rocuronium. ） The degree of saturation of AchR with different concentrations of rocuronium at each neuromuscular junction was calculated by （AchR0 - AchRE）/AchR0 which reflects the affinity of AchR with the rocuronium in orbicular muscle of mouth and gastrocnemius muscle. Results The density of AchR was significantly lower while the affinity with rocuronium was higher in gastrocnemius muscle than in orbicular muscle of mouth （ P 〈 0.05）. Conclusion The density of AchR is lower and the affinity of AchR at end-plate with rocuronium is significantly greater in gastrocnemius muscle innervated by sciatic nerve than in orbicular muscle of mouth innervated by facial nerve. This may explain the mechanism for different sensitivity of the muscles innervated by facial and peripheral nerves to rocuronium.

关 键 词：雄甾烷醇类 受体 胆碱能 面部肌肉 肌 骨骼 

分 类 号：R746.1[医药卫生—神经病学与精神病学]