消炎镇痛液混合牛痘疫苗致炎兔皮提取物对大鼠坐骨神经损伤的效应  

Effect of anti-inflAmmation-analgesic injection combined with extract from rabbit skin inflamed by vaccinia virus on sciatic nerve injury in rats

作　　者：魏淑明[1] 赵志辉[2] 陈晶[3] 王秀丽[1] 

机构地区：[1]河北医科大学第三医院麻醉科,石家庄市050051 [2]秦皇岛市海港医院麻醉科 [3]石家庄市中心医院麻醉科

出　　处：《中华麻醉学杂志》2011年第1期50-54,共5页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（30772082）;河北省自然科学基金（C2008000931）;河北省卫生厅资助课题（07090）

摘　　要：目的 评价消炎镇痛液混合牛痘疫苗致炎兔皮提取物（ERSVV）对大鼠坐骨神经损伤的效应.方法 成年SD大鼠50只,雌雄各半,体重260～300 g,采用随机数字表法,将大鼠随机分为5组（n=10）：假手术组（S组）、坐骨神经慢性压迫性损伤组（CCI组）、消炎镇痛液组（A组）、EBSVV组（E组）和混合用药组（A＋E组）.CCI组、A组、E组和A＋E组制备大鼠坐骨神经慢性压迫性损伤模型;S组仅暴露坐骨神经.在坐骨神经旁置管,于术后14 d开始给药,A组注射消炎镇痛液0.2Ml[地塞米松棕榈酸酯0.05ml（4 mg/ml）、2%利多卡因0.05 ml、维生素B120.05 ml（0.5 mg/ml）和注射用水0.05ml）];E组注射ERSVV 0.2ml（40 U/ml）;A＋E组注射消炎镇痛液（去掉注射用水0.05 ml）＋ERSVV0.05 ml共0.2 ml.第1天给药后,地塞米松棕榈酸酯改为注射用水,持续给药14 d.分别于术前1 d（基础状态）、术后1、5、7、14、21、28 d时测定机械缩足阈值（MWT）.5组分别于术后14、28 d时取5只大鼠,暴露坐骨神经,测定运动神经传导速度（NCV）及复合肌肉运动电位波幅（AP）,然后取结扎处坐骨神经,光镜下观察病理学结果,计数轴突数,并测定髓鞘厚度.结果 与S组比较,CCI组MW、AP、轴突计数和髓鞘厚度降低,NCV减慢（P＜0.01）;与CCI组比较,A组和E组MWT、AP、轴突计数和髓鞘厚度升高,NCV增快（P＜0.05）;与A组或E组比较,A＋E组MWT、AP、轴突计数和髓鞘厚度升高,NCV增快（P＜0.05或0.01）.结论 与消炎镇痛液或ERSVV比较,两种药物混合应用可进一步减轻大鼠慢性压迫性坐骨神经损伤.Objective To investigate the effect of anti-inflammation-analgesic injection （AIAI） combined with extract from rabbit skin inflamed by vaccinia virus （ERSVV） on repair of the damaged sciatic nerve in rats.Methods Fifty adult SD rats of both sexes weighing 260-300 g were randomly divided into 5 gronps （ n = 10each）： sham operation group （group S）; sciatic nerve chronic constriction injury group （group CCI）; CCI ＋ AIAI group （group A）; CCI ＋ ERSVV group （group E） and CCI ＋ AIAI ＋ ERSVV group （group A ＋ E）. Right sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4/0 catgut in CCI,A,E and A ＋ E groups. A catheter was placed around sciatic nerve, and fixed to the nearby muscle and kept unclogged by injecting 0.2 nl distilled water daily. AIAI and/or ERSVV 0.2 ml were injected via the catheter starting from the 14th day after operation. AIAI 0.2 ml contained dexamethasone palmitate （4 mg/ml） 0.05 ml, 2% lidocaine 0.05 ml and vitamin B12 （0.5 mg/ml） 0.05 ml in distilled water. Dexamethasone palmitate was omitted in AIAI starting from the 2nd of drug administration in group A and A ＋ E. Paw withdrawal threshold to mechanical stimulation （MWT） was measured before （baseline） and at 1, 5, 7, 14, 21 and 28 days after operation. Sciatic nerves were exposed at 14 and 28 days after operation in 5 rats in each group. Conduction velocity of motor nerve （NCV） and action potential （AP） of gastrocnemius muscle were measured. Sciatic nerve at the site of CCI was examined for pathologic changes, the number of axons （NA） and thickness of myelin sheath （TMS） with light microscope. Results CCI significantly decreased MWT, AP, NA, TMS and NCV in group CCI as compared with group S （P 〈0.01）. AIAI and/or ERSVV significantly attenuated CCI-induced decrease in MWT, AP, NA, TMS and NCV in A,E and A ＋ E groups as compared with CCI group （ P 〈 0.05）. Their curative effects were potentiated by combine

关 键 词：药物疗法 联合 痘苗病毒 皮肤 生物制品 坐骨神经病 

分 类 号：R651.3[医药卫生—外科学]