犬瘟热病毒甘肃株的分离和N蛋白基因遗传进化分析  

Isolation and Nucleoprotein Genetic Analysis of Canine Distemper Virus in Gansu

作　　者：王旭荣[1,2] 王小辉[1,2] 韩富杰 李世宏[1,2] 李建喜[1,2] 孟嘉仁[1,2] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [2]中国农业科学院临床兽医学研究中心,兰州730050 [3]兰州恒泰动物医院,兰州730050

出　　处：《西北农业学报》2011年第3期15-19,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(BRF080302)

摘　　要：采用Vero细胞从甘肃2008-2009年疑似犬瘟热感染病犬体内分离到8株病毒,通过RT-PCR方法扩增M蛋白基因初步鉴定所获得毒株均为犬瘟热病毒（CDV）。进一步通过RT-PCR方法扩增分离株的N蛋白基因,并进行序列分析。结果表明,8株分离株均为CDV,它们之间N蛋白基因的核苷酸同源性和推定的氨基酸同源性分别为98.4%-99.7%、98.3%-99.6%;8株CDV分离株的N蛋白基因和TN、A75/17相应序列的核苷酸同源性分别为98.5%-99.5%、97.0%-98.0%,推定的氨基酸同源性分别为98.3%-99.2%、98.7%-99.8%;8株CDV分离株N蛋白基因和疫苗株（Onderstepoort和Snyder Hill）的相应序列的核苷酸同源性和推定的氨基酸同源性分别为93.0%-94.0%、96.8%-98.3%。根据N蛋白基因所建立的遗传系统发育树可知这8株CDV分离株与TN的亲缘关系较近,而与疫苗株处于不同的分支上。说明从甘肃分离的CDV野毒株和TN由同一毒株演化而来,与疫苗株亲缘关系较远。8 isolates of canine distemper virus（CDV） were isolated from domestic dogs in Gansu province.The sequence amplification of membrane protein gene showed that these isolates were CDV isolates.Nucleoprotein gene of 8 CDV isolates shared 98.4%-99.7% nucleotide homology and 98.3%-99.6% amino acid homology with each other.The N gene of 8 CDV V isolates shared 98.5%-99.5%,97.0%-98.0% nucleotide homology,and 98.3%-99.2%,98.7%-99.8% amino acid identity with that of TN,A75/17 respectively.However,The Nucleoprotein gene of 8 CDV V isolates shared 93.0%-94.0% nucleotide homology and 96.8%-98.3% amino acid identity with that of vaccine stain（Onderstepoort and Snyder Hill）.The phylogenetic tree suggested that the strains from different provinces had close relationship,which were evolved by same strain.

关 键 词：犬瘟热病毒 N蛋白基因 遗传进化 

分 类 号：S852.659.5[农业科学—基础兽医学]