体外压力刺激对大鼠筋膜组织成纤维细胞形态和MMP-1、MMP-3合成释放影响的研究  

The Effect of Stress on Synthesis and Release of Cell Morphology, MMP-1 and MMP-3 by rat "Zusanli" Fascia Fibroblasts in vitro

作　　者：陈波[1] 王兴桂[1] 李小玉[2] 黄小瑾[1] 李佳霖[1] 

机构地区：[1]贵阳中医学院针灸推拿系,贵州贵阳550002 [2]四川大学华西口腔医学院生物医学工程教育部重点实验室,四川成都600041

出　　处：《辽宁中医杂志》2011年第4期770-772,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(30801490);贵州省科技厅科技基金项目(黔科合J字2006[2063]号)

摘　　要：目的:通过体外实验探讨压力刺激对大鼠筋膜组织成纤维细胞形态和MMP-1、MMP-3合成释放变化的影响,为推拿基本力学刺激"舒筋散结"的治疗作用提供细胞生物学方面的实验依据。方法:体外培养大鼠筋膜组织成纤维细胞,对细胞实施100KPa和200KPa压力刺激后,观察细胞形态的变化和检测细胞MMP-1、MMP-3合成释放的变化。结果:筋膜组织成纤维细胞接受压力作用后,100KPa压力组细胞发生回缩,细胞间隙加大,200KPa压力组出现回缩成圆形并漂浮的细胞,细胞形态缩小;100KPa和200KPa压力组MMP-1合成释放呈增加趋势,但均无显著性差异(P>0.05;P>0.05);MMP-3合成释放减少,差异均有统计学意义(P<0.01;P<0.05)。结论:压力刺激对筋膜组织成纤维细胞MMP-1、MMP-3合成释放的影响和对细胞形态的影响,可能是推拿良性发挥"舒筋散结"作用的细胞生物力学原理之一。Objective：To supply experimental bases from the view of cellular biomechanics for the basical mechanical principle of massage ＂relieving rigidity of muscles and resolving hard mass＂ by exploring the research of stress on synthesis cell morphology and release of MMP-1 and MMP-3 by rat＂Zusanli＂fascia fibroblasts in vitro.Methods：Rat＂Zusanli＂Point fascia fibroblast cells were cultured in vitro.100 kPa and 200 kPa strain was taken on cells respectively.Cell morphology were observed and MMP-1 and MMP-3 of extracelluar nutrient solution were examined.Results：After being stressed,cells of group of 100Kpa strain rebound and intercellular space gets wider.While for 200Kpa group,cells rebound and become round,the volume gets smaller.The synthesis and release of MMP-1 of both groups of cells tend to increase,but the differences has no statistical significance（P0.05,P0.05）.As to MMP-3,the synthesis and release increase,it has statistical significance（P0.01,P0.05）.Conclusion：Pressure stimulation on regulation of synthesis and release of MMP-1 and MMP-3 of acupoints fibroblast cells and the influence on cells morphology may be one of the celluar biomechanical principles of optimum ＂relieving rigidity of muscles and resolving hard mass＂of massage.

关 键 词：推拿 压力刺激 筋膜组织 成纤维细胞 舒筋散结 细胞形态 MMP-1 MMP-3 

分 类 号：R-33[医药卫生]