黏萼蝇子草蜕皮甾酮类成分的分离与鉴定  

Isolation and identification of ecdysterones from root of Silene viscidula Franch.

作　　者：金艺淑[1,2] 徐伟[1] 李玉山[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳医学院沈洲医院,辽宁沈阳110002

出　　处：《沈阳药科大学学报》2011年第4期269-271,282,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的对黏萼蝇子草根的化学成分进行研究。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱和反相半微量制备型高效液相色谱分离纯化,根据ESI-MS、1H-NMR和13C-NMR等谱学数据进行结构鉴定。结果从黏萼蝇子草根的乙醇提取物分离得到5个蜕皮甾酮类化合物,分别鉴定为20-羟基蜕皮激素(20-hydroxyecdysone,1)、1-epi-integristerone A(2)、abutasterone(3)、旌节花甾酮A(stachysterone A,4)和15-羟基旌节花甾酮A(15-hydroxystachysterone A,5);另外得到胡萝卜苷(daucosterol,6)和β-谷甾醇(β-sitosterol,7)2个甾醇类化合物。结论化合物2-5为蝇子草属植物中首次分离的化合物。Objective To study the constituents of the extract from the root of Silene viscidula Franch.Methods The chemical constituents were isolated by silica gel column,open ODS column,Sephadex LH-20 column chromatography,and semi-preparative HPLC.Chemical characters and spectroscopic analysis were employed for the structural identification.Results Five ecdysterone compounds were obtained and their structures were identified as 20-hydroxyecdysone（1）,1-epi-integristerone A（2）,abutasterone（3）,stachysterone（4）and 15-hydroxystachysterone A（5）,along with two sterol compounds daucosterol（6）and β-sitosterol（7）.Conclusions Compounds 25 are obtained from the genus Silene for the first time.

关 键 词：黏萼蝇子草 化学成分 蜕皮甾酮 甾醇 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284[医药卫生—药学]