抗坏血酸对人肝癌细胞增殖与再分化的作用(英文)  

Effects of ascorbic acid on human hepatoma cell proliferation and redifferentiation

作　　者：康九红[1] 石益民[1] 郑荣梁[1] 

机构地区：[1]兰州大学生物系,兰州中国730000

出　　处：《中国药理学报》1999年第11期1019-1024,共6页Acta Pharmacologica Sinica

摘　　要：目的:测定抗坏血酸(AA)对人肝癌细胞增殖与再分化的作用。方法:维甲酸(Tre)为阳性对照,测定细胞增殖、细胞表面电荷、生化变化和软琼脂细胞生长等指标。结果:用AA6mmol·L^(-1)处理后,肝癌细胞的生长和分裂指数显著下降,增殖抑制率达58.9％。与恶化有关的指标显著减轻,如细胞表面电荷明显降低,电泳率从1.64降到0.93μm·s^(-1)·V^(-1)·cm^(-1),甲胎蛋白(α-FP)由302μg·g^(-1)(protein)降为90,γ-谷氨酰转氨酶(γ-GT)活性由0.81U·g^(-1)(protein)降到0.16。酪氨酸-α-酮戊二酸转氨酶(TAT)活性由10.3μmol·g^(-1)(protein)升高为41.2,细胞克隆形成力降低94.4％。结论:AA能够抑制人肝癌细胞增殖,诱导分化,并逆转恶性表型。AIM: To examine the effects of ascorbic acid (AA) on hepatoma. METHODS:Choosing an all-trans tretinoin (Tre) as a positive control, cell growth, and cell redifferentiation tests by cell surface charges, biochemical changes, and cell growth in soft agar were measured. RESULTS: After being treated with AA 6 mmol· L-1, the growth curve and mitotic index of human hepatoma cells decreased remarkably, the cellular growth inhibitory rate amounted to 58.9 % . The indices related with cell malignancy alleviated, such as cell surface charge obviously decreased, the electrophoresis rate dropped from 1.64 μm·s-1 ·V-1 ·cm -1 to 0.93, the average value of α-fetoprotein (α-FP) content decreased from 302μg · g-1 (protein) to 90, and γ-glutamyl-transpeptidase (γ-GT) activity from 0.81 U ·g -1 (protein) to 0.16. The index related with cell differentiation increased, such as the average level of tyrosine-α-ketoglutarate transaminase activity increased from 10.3μmol·g-1 (protein) to 41.2, and the colonogenic potential decreased 94.4 %. CONCLUSION: AA can inhibit human hepatoma cells proliferation, induce redifferentiation, and reverse its malignant phenotypic characteristics.

关 键 词：抗坏血酸 维甲酸 肝细胞癌 癌细胞增殖分化 

分 类 号：R977.23[医药卫生—药品]