豆制品中7种防腐剂的高效液相色谱法同时测定  

Simultaneous determination of seven preservatives in soybean products by high-performance liquid chromatography with ultraviolet detection

作　　者：方波[1] 徐明敏[2] 陈波[2] 

机构地区：[1]浙江省宁波市镇海区疾病预防控制中心,浙江宁波315200 [2]浙江省宁波市北仑区疾病预防控制中心,浙江宁波315800

出　　处：《中国卫生检验杂志》2011年第4期803-805,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立高效液相色谱法同时测定豆制品中苯甲酸、山梨酸、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯等7种食品防腐剂的分析方法。方法:样品经热水提取后,采用C18色谱柱,以0.02 mol/L的乙酸铵和甲醇为流动相,采用梯度洗脱分离,在紫外235 nm波长进行检测。结果:7种食品防腐剂的加标回收率在87.0%~99.6%范围,其相对标准偏差均小于5.0%,在0.1 mg/L^50.0 mg/L范围均具有良好的线性,其回归系数均大于0.999,检出限(LOD)在0.2 mg/kg^0.5 mg/kg范围。结论:本方法简便、灵敏、重现性好,能满足豆制品中防腐剂残留的监测要求。Objective：To establish a method for the simultaneous determination of seven preservatives including benzoic acid,sorbic acid,dehydroacetic acid,methylparaben,ethylparaben,propyl p-hydroxybenzoate,butyl p-hydroxybenzoate in soybean products by high-performance liquid chromatography.Methods： After the analytes were extracted with hot water,the sample was separated on a C18 column by a mobile phase consisting of 0.02 mol/L ammonium acetate/methanol in gradient elution.Detection was carried out by an ultraviolet detector at 235 nm.Results： Calibration curves of the seven preservatives were linear within the range of 0.1 mg/L^50.0 mg/L with correlation coefficients of greater than 0.999,and the limits of detection（LOD） were between 0.2 mg/kg^0.05 mg/kg.The extraction recoveries were between 87.0%~99.6%,and the RSDs were less than 5.0%.Conclusion： This established method is simple,sensitive,accurate and specific for monitoring of the seven preservatives residues in soybean products.

关 键 词：液相色谱法 豆制品 防腐剂 残留测定 

分 类 号：O657.7[理学—分析化学]