高效液相色谱法测定化妆品中7种防腐剂  

Determination of seven preservatives in cosmetics by high performance liquid chromatography

作　　者：侯逸众[1] 黄方取 严继东[1] 金良正 

机构地区：[1]浙江省金华市疾病预防控制中心,浙江金华321002

出　　处：《中国卫生检验杂志》2011年第4期832-833,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立高效液相色谱法测定化妆品中苯甲醇、苯甲酸、苯氧乙醇、对羟基苯甲酸甲酯、对羟基苯甲酸乙脂、对羟基苯甲酸异丙酯、对羟基苯甲酸丙酯7种防腐剂的方法。方法：采用Phenomenex Gemini C18柱（250 mm×46 mm×5μm）为分析柱,乙腈＋0.05 mol/L磷酸二氢钠（磷酸调节pH值为3.5）为流动相,梯度洗脱,检测波长260 nm,方法相对《化妆品卫生规范》更简单。结果：方法回收率为91.2%-103.1%,色谱峰面积相对标准偏差为1.47%-2.87%。结论：本法操作简单,灵敏度高,线性好,适合化妆品中防腐剂的测定。Objective：To determine seven preservatives in cosmetics,including benzyl alcohol,benzoic acid,2-pshenoxyehtanol,methyl p-hydroxy benzoate,ethyl p-hydroxy benzoate,4-hydroxybenzoic acid iso-propyl ester,propyl-p-hydroxy benzoate by high performance liquid chromatographic method.Methods： A Phenomenex Gemini C18（250 mm×46 mm×5 μm） column was used with a mobile phase consist of acetonitrile,0.05 mol/L NaH2 PO4（pH=3.5,adjusted by phosphate acid） and gradient elution.The wavelength 260 nm was selected.Compared to Hygienic Standard for Cosmetics,the method is simple.Results： The recovery lies within a range of 91.2%~103.1% and the relative standard deviation 1.47%~2.87%.Conclusion： The method discussed is simple with high sensitivity and good linearity,suitable for the determination of preservatives in cosmetics.

关 键 词：化妆品 高效液相色谱 防腐剂 

分 类 号：O657.7[理学—分析化学]