蛹虫草子实体的多糖含量测定研究  

A study on determination of polysaccharides in sporophore of cordyceps militaris

作　　者：蔡晓[1] 汤晓勤[1] 杨长晓[1] 

机构地区：[1]四川省疾病预防控制中心,成都610041

出　　处：《中国卫生检验杂志》2011年第4期837-838,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨蛹虫草子实体中多糖的提取和含量的紫外分光光度测定方法。方法:采用水提醇沉再经过碱性硫酸铜纯化的方法进行制备,多糖在强酸性条件水解成单糖分子,在沸水浴中脱水生成羟甲基呋喃甲醛(羟甲基糖醛)再与苯酚缩合成淡橙黄色的化合物,其显色强度与溶液中单糖的浓度成正比,灵敏而稳定,比色测定溶液在485 nm波长处有最大吸收。结果:对照品葡聚糖计的检测浓度在0.0 mg/ml^0.1 mg/ml范围内与吸光度呈良好的线性关系,得回归方程y=9.7717x-0.0006r=0.9996,平均回收率97.37%,RSD为2.73%。结论:紫外分光光度法灵敏、快速、准确、成本低。适用于蛹虫草子实体中多糖含量测定。在制备测定液中提取方法不同,多糖的测定值也所差异。Objective：To investigate extraction and ultraviolet spectrophotometric determination of polysaccharides in sporophore of Cordyceps militaris（L.） link.Methods： The sample was processed by extraction using water,precipitation using ethanol and purification using basic cupric sulfate.Polysaccharides,under strong acidic conditions,were hydrolyzed into monosaccharide molecules.Dehydration in a boiling water bath produced hydroxymethyl furfural,which was subjected to condensation with phenol to produce a compound with light orange color.The color intensity was in a sensitive and stable positive relationship with the concentration of monosaccharides in the solution and exhibited the maximum absorption at 485 nm in colorimetric determination.Results： Good linearity was presented between the absorbance value and the concentration of the reference substance（calculated with respect to polyglucosan） within the range of 0.0 mg/ml^0.1 mg/ml.The regression equation was y=9.7717x-0.0006（r=0.9996）.The mean recovery was 97.37% and the RSD was 2.73%.Conclusion： Ultraviolet spectrophotometry is sensitive,rapid,stable and cost-effective and thus applicable to determination of polysaccharides in sporophore of Cordyceps militaris（L.） link.Different extraction methods may lead to different results.

关 键 词：蛹虫草 葡聚糖 紫外分光光度法 含量测定 

分 类 号：O657.32[理学—分析化学]