头孢菌素C去乙酰基酶产生菌的筛选、酶学性质及产酶条件优化  

Screening and identification of CAH-producing strain, enzyme properties of cephalosporin-C deacetylase and optimization of enzyme production

作　　者：韩云宾[1] 周文瑜[1] 胡媛[1] 魏东芝[1] 沈亚领[1] 方善综 金雄熊 叶凤起 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,鲁华生物技术研究所,上海200237 [2]浙江永宁药业股份有限公司,浙江台州318020

出　　处：《工业微生物》2011年第2期1-6,共6页Industrial Microbiology

基　　金：上海市重点学科建设项目资助(B505)

摘　　要：以7-ACA为底物从土壤中筛选得到一株产头孢菌素C乙酰水解酶的微生物,初步鉴定为红酵母。酶学性质研究表明:静息细胞CAH的最适pH为7.0,最适反应温度为25℃;在温度低于40℃保存静息细胞时CAH的稳定性很好,在pH 5.5～8.0的范围内保存静息细胞时CAH比较稳定。产酶条件优化结果为:葡萄糖30g/L,国产酵母粉20 g/L,KH_2PO_4 5g/L,培养基的最优初始pH为7.0。When 7-aminocephalosporanic acid （7-ACA） was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, a strain producing CAH with high yield was obtained. It was identified as red yeast. The enzyme was most active at 25℃ and pH 7.0. The enzyme was stable at temperatures lower than 40℃ and within pH 5.5-8.0. The optimized conditions for enzyme production were as followings： glucose 30 g/ L, homemade yeast 20 g/L and KH2PO45 g/L, while the initial medium pH was 7.0.

关 键 词：7-氨基头孢烷酸 去乙酰基-7-氨基头孢烷酸 头孢菌素C乙酰水解酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]