融粘帚霉对葛根素的生物转化研究  

Biotransformation of puerarin into 3'-hydroxyrjuerarin by Gliocladiwn deliguescens

作　　者：杨莉萍[1] 崔志超[1] 高芳[1] 张新[1] 叶辉[1] 

机构地区：[1]南通大学生命科学学院,南通226007

出　　处：《工业微生物》2011年第2期47-50,共4页Industrial Microbiology

基　　金：江苏省南通市科技计划财政资金资助项目(K2009050)

摘　　要：采用融粘帚霉AS3.3987对葛根素进行微生物转化,生成的主要产物经分析鉴定是3′-羟基葛根素。采用高效液相色谱方法,以XDB-C18为色谱柱,甲醇:水(27:73)为流动相,检测波长为254 nm,测得在160 r/min、30℃的转化条件下,底物加量为400μg/mL,转化时间为36 h时,3'-羟基葛根素得率最高为93.2 mg/g葛根素。The major product of microbial transformation of puerarin by Gliocladium deliguescens AS3. 3987 was identi- fied as 3＇-hydroxypuerarin on the basis of its spectral data. 3＇-hydroxypuerarin was directly determined by HPLC. Methanol and water （27：73） was used as the mobile phase on C18 column and the UV detection wavelength was 254 nm. The maximum transformation rate obtained was 93.2 mg 3＇-hydroxypuerarin/g puerarin at 30℃ 160 r/min for 36 h with the substrate concentration of 400μg/mL.

关 键 词：高效液相色谱法 微生物转化 葛根素 3’-羟基葛根素 融粘帚霉 

分 类 号：TQ920[轻工技术与工程—发酵工程]