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机构地区:[1]广州体育学院运动与健康系,广东广州510500
出 处:《北京体育大学学报》2011年第3期56-58,共3页Journal of Beijing Sport University
基 金:2009年广州体育学院院管课题:线粒体电子漏与运动性疲劳的产生及其消除的分子机制(课题编号:YB0923)
摘 要:目的:探讨高住低练对线粒体钙转运功能及能量代谢的影响,为运动与低氧适应机制的研究和高原训练方案的制定提供理论依据。方法:采用荧光技术和生物发光技术分别测定心肌、肝脏和骨骼肌线粒体Ca2+浓度和心肌线粒体ATP含量,并用血乳酸分析仪和血糖仪分别测定血乳酸和血糖浓度。结果:急性低氧应激使线粒体游离Ca2+浓度和ATP含量下降,血乳酸明显上升;经过高住低练运动与低氧适应后线粒体游离Ca2+浓度和ATP含量呈上升趋势,血乳酸上升程度减低,提示依赖糖酵解产能降低,能量代谢产生了适应。结论:高住低练能使线粒体钙转运功能加强,能量代谢作用得到改善。Aim: The effects of HiLo training on the function of mitochondria calcium transports and energy metabolism were investigated,so that the theory of study on the mechanisms of hypoxic acclimatization and project of high altitude training is provided.Methods: The Ca2+concentration of mitochondria in the heart, liver and skeletal muscle tissue and ATP content were assessed by fluorescence and bioluminescence techniques,and blood lactate and blood glucose concentration were measured by blood lactate analyzer and glucose meters.Results: Free Ca2+concentration,ATP content of mitochondria decreased after hypoxic stress and blood lactic acid increased;Free Ca2+concentration,ATP content of mitochondria increased after 4-week hypoxic acclimatization at altitude 3 000 m and significantly after hypoxic training acclimatization,blood lactic acid increasing a little shows that it depending on energy of glycolytic decreased,energy metabolism and energy metabolism developed adaptation.Conclusion: The function of mitochondria calcium transports increased and energy metabolism was improved after living high-training low.
分 类 号:G804.7[文化科学—运动人体科学]
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